Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint

• Published in: Science (American Association for the Advancement of Science) Vol. 279; no. 5353; pp. 1045 - 1047
• Main Authors: Kim, Sang Hoon, Lin, Diana P., Matsumoto, Sanae, Kitazono, Ana, Matsumoto, Tomohiro
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 13.02.1998; American Association for the Advancement of Science
• Subjects: Amino acids; Anaphase; Anaphase-Promoting Complex-Cyclosome; Biological and medical sciences; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cdc20 Proteins; Cell Cycle Proteins; Cell cycle, cell proliferation; Cell physiology; Chromosomes; Chromosomes, Fungal - metabolism; Diploidy; DNA Replication; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes, Fungal; Genetic mutation; Haploidy; Kinesin - genetics; Kinesin - metabolism; Ligases - metabolism; Mad2 Proteins; Metaphase; Mitosis; Mitotic spindle apparatus; Molecular and cellular biology; Mutagenesis, Site-Directed; Mutation; Nuclear Proteins; Plasmids; Polymerase chain reaction; Recombinant Fusion Proteins - metabolism; Schizosaccharomyces - cytology; Schizosaccharomyces - genetics; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe Proteins; Spindle (Cell division); Spindle (Cytoplasm); Spindle Apparatus - metabolism; Transformation, Genetic; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Yeasts; Yeast; Mitosis; Cell division; Molecular interaction; Mitotic spindle; Fungi; Regulation(control); Shutdown; Gene; Cell cycle; Schizosaccharomyces pombe; Ascomycetes; Mutation; Thallophyta
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.279.5353.1045

Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.