A three-dimensional in vitro culture environment of a novel polymer scaffold, yielding chondroprogenitors and mesenchymal stem cells in human chondrocytes derived from osteoarthritis-affected cartilage tissue

• Published in: Journal of Orthopaedics Vol. 23; pp. 138 - 141
• Main Authors: Katoh, Shojiro, Yoshioka, Hiroshi, Iwasaki, Masaru, Senthilkumar, Rajappa, Rajmohan, Mathaiyan, Karthick, Ramalingam, Preethy, Senthilkumar, Abraham, Samuel JK
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.01.2021; Elsevier BV; Elsevier
• Subjects: CD49e; Chondrocytes; Chondroprogenitors; Mesenchymal stem cells; Orthopedics; Osteoarthritis; Thermo-reversible gelation polymer (TGP); CD49e; Chondroprogenitors; Thermo-reversible gelation polymer (TGP); Osteoarthritis; Chondrocytes; Mesenchymal stem cells
• ISSN: 0972-978X; 0972-978X
• DOI: 10.1016/j.jor.2021.01.005

We evaluated the expression of stem/progenitor biomarkers in osteoarthritic tissue derived chondrocytes cultured using a three-dimensional (3D) thermo-reversible gelation polymer (TGP). The chondrocytes from discarded biopsy tissues obtained from human elderly patients with osteoarthritis were cultured using the 3D-TGP up to six weeks. The chondrocytes grew in a tissue-like manner, without de-differentiation into fibroblasts, and the cells thus tissue-engineered were proven positive for CD49e, OCT4, CD-105 and STRO-1 by immunohistochemistry. This study establishes the efficacy of this 3D-TGP platform for clinically useable in-vitro tissue-engineered cartilage for improvising the clinical outcome of cell therapy for cartilage repair.