Propofol induces proliferation and invasion of gallbladder cancer cells through activation of Nrf2

• Published in: Journal of experimental & clinical cancer research Vol. 31; no. 1; p. 66
• Main Authors: Zhang, Lingmin, Wang, Ning, Zhou, Suna, Ye, Wenguang, Jing, Guixia, Zhang, Mingxin
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.08.2012; BioMed Central; BMC
• Subjects: Anesthesiology; Animals; Apoptosis; Apoptosis - drug effects; Cancer; Cancer cells; Cell culture; Cell Line, Tumor; Cell Proliferation - drug effects; Experiments; Gallbladder cancer; Gallbladder Neoplasms - genetics; Gallbladder Neoplasms - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Gene Knockdown Techniques; Human acts; Human behavior; Humans; Invasion; Mice; Neoplasm Invasiveness - prevention & control; NF-E2-related factor 2 (Nrf2); NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Phenols; Proliferation; Propofol; Propofol - administration & dosage; Proteins; Studies
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/1756-9966-31-66

Propofol is one of the most commonly used intravenous anaesthetic agents during cancer resection surgery, but the effect of propofol on gallbladder cancer is not clear. NF-E2-related factor 2 (Nrf2) is abundantly expressed in cancer cells and relates to proliferation, invasion, and chemoresistance. The aims of the current study were to evaluate effects of propofol on the behavior of human GC cells and role of Nrf2 in these effects. The effects of propofol on cell proliferation, apoptosis, and invasion were detected by MTT assays, flow cytometry, and transwell assay. Also, activation of Nrf2 was determined by western blot, RT-PCR, and immunofluorescence assays. Nrf2 was knocked-down in GBC-SD cells by shRNA before evaluating the role of Nrf2 in the influence of propofol on biological behaviors. Propofol promoted the proliferation of GBC-SD cells in a dose- and time- dependent manner. After exposure to propofol for 48 h, GBC-SD cells showed decreased apoptosis and increased invasion. Also, propofol over-expressed Nrf2 at both the protein and mRNA levels and induced translocation of Nrf2 into the nucleus. Finally, loss of Nrf2 by shRNA reversed the effect of propofol on cell proliferation, apoptosis, and invasion. Propofol induces proliferation and promotes invasion of GC cells through activation of Nrf2.