Phylogenetic comparisons reveal mosaic histories of larval and adult shell matrix protein deployment in pteriomorph bivalves

• Published in: Scientific reports Vol. 10; no. 1; pp. 22140 - 12
• Main Authors: Zhao, Ran, Takeuchi, Takeshi, Koyanagi, Ryo, Villar-Briones, Alejandro, Yamada, Lixy, Sawada, Hitoshi, Ishikawa, Akito, Iwanaga, Shunsuke, Nagai, Kiyohito, Che, Yuqi, Satoh, Noriyuki, Endo, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.12.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 631/181/2468; 631/181/735; 631/337/475; 704/445/330; Animal Shells - metabolism; Animals; Bivalvia; Calcium carbonate; Cambrian; Carbonic anhydrases; Chitin; Crassostrea - classification; Crassostrea - genetics; Divergence; Evolution; Evolution & development; Evolution, Molecular; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Humanities and Social Sciences; Larva; Matrix protein; Mineralization; Mollusks; Mosaicism; multidisciplinary; Oysters; Phylogenetics; Phylogeny; Pinctada - classification; Pinctada - genetics; Polysaccharides; Proteome - metabolism; Proteomics - methods; Saccharides; Science; Science (multidisciplinary); Von Willebrand factor
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-79330-x

Molluscan shells are organo-mineral composites, in which the dominant calcium carbonate is intimately associated with an organic matrix comprised mainly of proteins and polysaccharides. However, whether the various shell matrix proteins (SMPs) date to the origin of hard skeletons in the Cambrian, or whether they represent later deployment through adaptive evolution, is still debated. In order to address this issue and to better understand the origins and evolution of biomineralization, phylogenetic analyses have been performed on the three SMP families, Von Willebrand factor type A (VWA) and chitin-binding domain-containing protein (VWA-CB dcp), chitobiase, and carbonic anhydrase (CA), which exist in both larval and adult shell proteomes in the bivalves, Crassostrea gigas and Pinctada fucata . In VWA-CB dcp and chitobiase, paralogs for larval and adult SMPs evolved before the divergence of these species. CA-SMPs have been taken as evidence for ancient origins of SMPs by their presumed indispensable function in biomineralization and ubiquitous distribution in molluscs. However, our results indicate gene duplications that gave rise to separate deployments as larval and adult CA-SMPs occurred independently in each lineage after their divergence, which is considerably more recent than hitherto assumed, supporting the “recent heritage and fast evolution” scenario for SMP evolution.