One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia

• Published in: PLoS neglected tropical diseases Vol. 13; no. 6; p. e0007421
• Main Authors: Barkham, Timothy, Zadoks, Ruth N., Azmai, Mohammad Noor Amal, Baker, Stephen, Bich, Vu Thi Ngoc, Chalker, Victoria, Chau, Man Ling, Dance, David, Deepak, Rama Narayana, van Doorn, H. Rogier, Gutierrez, Ramona A., Holmes, Mark A., Huong, Lan Nguyen Phu, Koh, Tse Hsien, Martins, Elisabete, Mehershahi, Kurosh, Newton, Paul, Ng, Lee Ching, Phuoc, Nguyen Ngoc, Sangwichian, Ornuma, Sawatwong, Pongpun, Surin, Uraiwan, Tan, Thean Yen, Tang, Wen Ying, Thuy, Nguyen Vu, Turner, Paul, Vongsouvath, Manivanh, Zhang, Defeng, Whistler, Toni, Chen, Swaine L.
• Format: Journal Article
• Language: English
• Published: United States PLoS 01.06.2019; Public Library of Science; Public Library of Science (PLoS)
• Subjects: Adolescent; Adult; Adults; Aged; Aged, 80 and over; Analysis; Animal health; Animals; Antimicrobial agents; Aquaculture; Aquaculture industry; Arthritis; Asia, Southeastern - epidemiology; Biology and Life Sciences; Causes of; Child; Child, Preschool; Clinical medicine; Cloning; Collaboration; Collections; Computer and Information Sciences; Disease control; Disease prevention; Disease transmission; Diseases; Distribution; DNA sequencing; Earth Sciences; Economics; Elderly; Environmental health; Epidemiology; Female; Fish; Fish Diseases - epidemiology; Fish Diseases - microbiology; Fish farms; Fisheries; Fishing; Foodborne diseases; Foodborne Diseases - epidemiology; Foodborne Diseases - microbiology; Freshwater; Freshwater fish; Freshwater fishes; Gene sequencing; Genetic aspects; Genomes; Genomics; Genotype; Hospitals; Humans; Identification and classification; Infant; Infant, Newborn; Infection; Infectious arthritis; Infectious diseases; Inland water environment; Invasive species; Laboratories; Literature reviews; Male; Medical research; Medicine and health sciences; Meningitis; Middle Aged; Molecular Epidemiology; Multilocus Sequence Typing; Neonates; Newborn infants; Older people; Outbreaks; People and Places; Phylogeny; Pregnancy; Pregnant women; Public health; Sepsis; Sequencing; Streptococcal Infections - epidemiology; Streptococcal Infections - microbiology; Streptococcal Infections - veterinary; Streptococcus agalactiae; Streptococcus agalactiae - classification; Streptococcus agalactiae - isolation & purification; Streptococcus agalactiae - pathogenicity; Streptococcus infections; Tilapia; Transmission; Tropical diseases; Veterinary medicine; Virulence; Virulence (Microbiology); Whole Genome Sequencing; Young Adult; Asia, Southeastern; Southeast Asia; Vietnam; Singapore; Cambodia; United Kingdom > UK; United States > US; Brazil; Malaysia; Thailand
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0007421

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Background: In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farmed freshwater fish. Although GBS sepsis is well-described in neonates and older adults with co-morbidities, this outbreak affected non-pregnant and younger adults with fewer co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been reported from twenty humans in Hong Kong and two in France, and from one fish in Thailand. We hypothesised that ST283 was causing region-wide infection in Southeast Asia. Methodology/principal findings: We performed a literature review, whole genome sequencing on 145 GBS isolates collected from six Southeast Asian countries, and phylogenetic analysis on 7,468 GBS sequences including 227 variants of ST283 from humans and animals. Although almost absent outside Asia, ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand, 4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and Vietnam, in seven fish species in Singapore markets, and a diseased frog in China. Conclusions: GBS ST283 is widespread in Southeast Asia, where it accounts for a large proportion of bacteraemic GBS, and causes disease and economic loss in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However, whether transmission is from aquaculture to humans, or vice versa, or involves an unidentified reservoir remains unknown. Creation of cross-border collaborations in human and animal health are needed to complete the epidemiological picture. Support for this project was provided by the Molecular Biology Laboratory and the Department of Laboratory Medicine, Tan Tock Seng Hospital; by the Environmental Health Institute, National Environment Agency, Singapore; by the Ministry of Health, Singapore, through the Singapore Infectious Diseases Initiative grant number SIDI/2016/002 (TB) https://www.moh.gov.sg, and the National Medical Research Council, Ministry of Health, Singapore grant number NMRC/CIRG/1467/2017 (SLC) http://www.nmrc.gov.sg, by the UK Global Challenges Research Fund via the Scottish Funding Council, SFC/AN/10/2018 (RNZ) http://www.sfc.ac.uk, and by the Global Disease Detection program of the U.S. Centers for Disease Control and Prevention.