Histone demethylase JMJD1A coordinates acute and chronic adaptation to cold stress via thermogenic phospho-switch

• Published in: Nature communications Vol. 9; no. 1; pp. 1566 - 16
• Main Authors: Abe, Yohei, Fujiwara, Yosuke, Takahashi, Hiroki, Matsumura, Yoshihiro, Sawada, Tomonobu, Jiang, Shuying, Nakaki, Ryo, Uchida, Aoi, Nagao, Noriko, Naito, Makoto, Kajimura, Shingo, Kimura, Hiroshi, Osborne, Timothy F., Aburatani, Hiroyuki, Kodama, Tatsuhiko, Inagaki, Takeshi, Sakai, Juro
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.04.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/106; 13/95; 14/28; 38/15; 38/70; 38/89; 38/91; 631/443/319/2723; 631/80/458/1733; 64/60; 82/29; 96; 96/95; Acclimatization; Adaptation; Adipogenesis; Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - enzymology; Adipose Tissue, Brown - metabolism; Adipose Tissue, White - enzymology; Adipose Tissue, White - metabolism; Adrenergic receptors; Animals; Browning; Cold; Cold Temperature; Cold-Shock Response; Demethylation; Female; Histone H3; Humanities and Social Sciences; Jumonji Domain-Containing Histone Demethylases - chemistry; Jumonji Domain-Containing Histone Demethylases - genetics; Jumonji Domain-Containing Histone Demethylases - metabolism; Kinases; Lysine; Male; Mammals; Metabolic disorders; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular modelling; multidisciplinary; Phosphorylation; PPAR gamma - genetics; PPAR gamma - metabolism; Science; Science (multidisciplinary); Signal Transduction; Signaling; Stress; Stresses; Thermogenesis; Uncoupling Protein 1 - genetics; Uncoupling Protein 1 - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-03868-8

In acute cold stress in mammals, JMJD1A, a histone H3 lysine 9 (H3K9) demethylase, upregulates thermogenic gene expressions through β-adrenergic signaling in brown adipose tissue (BAT). Aside BAT-driven thermogenesis, mammals have another mechanism to cope with long-term cold stress by inducing the browning of the subcutaneous white adipose tissue (scWAT). Here, we show that this occurs through a two-step process that requires both β-adrenergic-dependent phosphorylation of S265 and demethylation of H3K9me2 by JMJD1A. The histone demethylation-independent acute Ucp1 induction in BAT and demethylation-dependent chronic Ucp1 expression in beige scWAT provides complementary molecular mechanisms to ensure an ordered transition between acute and chronic adaptation to cold stress. JMJD1A mediates two major signaling pathways, namely, β-adrenergic receptor and peroxisome proliferator-activated receptor-γ (PPARγ) activation, via PRDM16-PPARγ-P-JMJD1A complex for beige adipogenesis. S265 phosphorylation of JMJD1A, and the following demethylation of H3K9me2 might prove to be a novel molecular target for the treatment of metabolic disorders, via promoting beige adipogenesis. JMJD1A is essential for thermogenic gene induction in brown adipose tissue. Here the authors show that white adipose tissue beige-ing requires both β-adrenergic-dependent phosphorylation of S265 and demethylation activity of JMJD1A while brown adipose tissue-driven thermogenesis requires β-adrenergic dependent phosphorylation of S265 but is independent of H3K9me2 demethylation.