Alpha tryptase allele of Tryptase 1 ( TPSAB1 ) gene associated with Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) in Vietnam and Philippines

• Published in: Human immunology Vol. 76; no. 5; pp. 318 - 323
• Main Authors: Vasquez Velasquez, Clara, Roman, Arthur Dessi, Lan, Nguyen Thi Phuong, Huy, Nguyen Tien, Mercado, Edelwisa Segubre, Espino, Fe Esperanza, Perez, Ma Lucila M, Huong, Vu Thi Que, Thuy, Tran Thi, Tham, Vo Dinh, Nga, Cao Thi Phi, Ha, Tran Thi Ngoc, Bilar, Josie M, Bajaro, Jemimah Dawn P, Baello, Benilda Q, Kikuchi, Mihoko, Yasunami, Michio, Morita, Kouichi, Watanabe, Naohiro, Karbwang, Juntra, Hirayama, Kenji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2015
• Subjects: Adolescent; Allergy and Immunology; Child; Child, Preschool; Chymase (CMA1); Chymases - genetics; Dengue Hemorrhagic Fever (DHF); Dengue Shock Syndrome (DSS); Dengue Virus - immunology; Female; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Homozygote; Humans; Male; Mast Cells - immunology; Mast Cells - virology; Philippines; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; Severe Dengue - genetics; Severe Dengue - immunology; Single nucleotide polymorphism (SNP); Tryptase 1 (TPSAB1); Tryptases - genetics; Vietnam; Vietnam; Philippines; Vietnam, Ho Chi Minh City; ISEW, Vietnam; ISEW, Philippines; Dengue Hemorrhagic Fever; TPSB2; DFD; Vinh Long Providence; single nucleotide polymorphism; TPSAB1PF; Philippine Children’s Medical Center; odds ratio; Dengue virus; TPSAB1PR; DV; RT-PCR; reverse transcription-PCR; Chymase ( CMA1); TPSAB1; IMC; RFLP; tryptase human gene alpha-beta 1; linkage disequilibrium; tryptase human gene alpha-beta 2; WHO; World Health Organization; DSS; Dengue fever; chymase gene forward primer; tryptase gene reverse primer; Ho Chi Minh City; p-value probability value; base pairs; Tryptase 1 ( TPSAB1); Integrated Management of Childhood Illness; VL; NK; Single nucleotide polymorphism (SNP); Dengue virus serotype; Ig; PCR; ELISA; CMA1PF; chymase human gene 1; DHF; CMA1PR; P; restriction fragment length polymorphism; SNP; RITM; Research Institute for Tropical Medicine; natural killer cell; polymerase chain reaction; OR; immunoglobulin; CMA1; chymase gene reverse primer; DEN; PCMC; Bp; deoxy-ribonucleic acid; HCMC; tryptase gene forward primer; Dengue Shock Syndrome (DSS); DNA; enzyme-linked immunosorbent assay; LD; Dengue Hemorrhagic Fever (DHF); Dengue Shock Syndrome; Chymase (CMA1); Tryptase 1 (TPSAB1)
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/j.humimm.2015.03.009

Abstract We previously reported, significantly higher levels of Chymase and Tryptase in early stage plasma of DSS patients prior to the occurrence of shock suggesting a possible role of mast cells in dengue pathogenesis. To further investigate, we analyzed CMA1 promoter SNP (rs1800875) and TPSAB1 gene alleles , which encode the Human Chymase and α- and β- tryptase 1 enzymes respectively, for susceptibility to Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) in patients from hospitals in Vietnam (Ho Chi Minh City and Vinh Long) and the Philippines. While the CMA1 promoter SNP (rs1800875) was not associated with DHF/DSS, the homozygous form of α-tryptase allele was associated with DSS patients in Vinh Long and the Philippines (OR = 3.52, p < 0.0001; OR = 3.37, p < 0.0001, respectively) and with DHF in Ho Chi Minh City (OR = 2.54, p = 0.0084). Also, a statistically significant association was observed when DHF and DSS were combined in Vinh Long (OR = 1.5, p = 0.034) and the Philippines (OR = 2.36, p = 0.0004); in Ho Chi Minh City when DHF and DSS were combine an association was observed, but it was not statistically significant (OR = 1.5, p = 0.0505). Therefore, the α-tryptase might have a possible effect on the susceptibility to severe form of Dengue infection.