Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients

• Published in: Scientific reports Vol. 11; no. 1; pp. 4500 - 4
• Main Authors: Yokota, Isao, Hattori, Takeshi, Shane, Peter Y., Konno, Satoshi, Nagasaka, Atsushi, Takeyabu, Kimihiro, Fujisawa, Shinichi, Nishida, Mutsumi, Teshima, Takanori
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.02.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2234; 631/326/596/4130; 692/699/255; Adult; COVID-19; COVID-19 - diagnosis; COVID-19 - virology; COVID-19 Testing - methods; Diagnostic Tests, Routine - methods; Female; Humanities and Social Sciences; Humans; Male; Medical personnel; Middle Aged; multidisciplinary; Nasopharynx - virology; Polymerase Chain Reaction - methods; RNA, Viral - genetics; Saliva; Saliva - virology; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Specimen Handling - methods; Viral Load - methods
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-84059-2

Emerging evidences have shown the utility of saliva for the detection of SARS-CoV-2 by PCR as alternative to nasopharyngeal swab (NPS). However, conflicting results have been reported regarding viral loads between NPS and saliva. We conducted a study to compare the viral loads between NPS and saliva in 42 COVID-19 patients. Viral loads were estimated by the cycle threshold (Ct) values. SARS-CoV-2 was detected in 34 (81%) using NPS with median Ct value of 27.4, and 38 (90%) using saliva with median Ct value of 28.9 (P = 0.79). Kendall’s W was 0.82, showing a high degree of agreement, indicating equivalent viral loads in NPS and saliva. After symptom onset, the Ct values of both NPS and saliva continued to increase over time, with no substantial difference. Self-collected saliva has a detection sensitivity comparable to that of NPS and is a useful diagnostic tool with mitigating uncomfortable process and the risk of aerosol transmission to healthcare workers.