Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor

• Published in: FEMS microbiology ecology Vol. 51; no. 2; pp. 247 - 256
• Main Authors: Nielsen, Michael, Bollmann, Annette, Sliekers, Olav, Jetten, Mike, Schmid, Markus, Strous, Marc, Schmidt, Ingo, Larsen, Lars Hauer, Nielsen, Lars Peter, Revsbech, Niels Peter
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Aerobiosis; Aggregates; Ammonia; Ammonia - metabolism; Anaerobic processes; Anaerobiosis; Anammox; Bacteria, Aerobic - metabolism; Bacteria, Anaerobic - metabolism; Bacteriology; Biological and medical sciences; Bioreactors - microbiology; CANON; Diffusion; Diffusional limitation; Ecology; Flow rates; Flow velocity; Fluorescence; Fluorescence in situ hybridization; Fundamental and applied biological sciences. Psychology; Kinetics; Microbiology; Microscale distribution; Miscellaneous; Nitrification; Nitrites - metabolism; Nitrogen removal; Organic chemistry; Oxidation; Oxidation-Reduction; Oxygen; Oxygen - metabolism; Reaction kinetics; Reactors; Microscale distribution; CANON; Anammox; Diffusional limitation; Kinetics; Nitrification; Molecular hybridization; Oxygen; Anoxia; Microbiology; Respiratory disease; In situ; Ecology; Aerobe; Biomass; Fluorescence in situ hybridization; Bacteria; Oxidation
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1016/j.femsec.2004.09.003

In the Completely Autotrophic Nitrogen removal Over Nitrite (CANON) process, aerobic and anaerobic ammonia oxidizing bacteria cooperate to remove ammonia in one oxygen-limited reactor. Kinetic studies, microsensor analysis, and fluorescence in situ hybridization on CANON biomass showed a partial differentiation of processes and organisms within and among aggregates. Under normal oxygen-limited conditions (∼5 μM O 2), aerobic ammonia oxidation (nitrification) was restricted to an outer shell (<100 μm) while anaerobic ammonia oxidation (anammox) was found in the central anoxic parts. Larger type aggregates (>500 μm) accounted for 68% of the anammox potential whereas 65% of the nitrification potential was found in the smaller aggregates (<500 μm). Analysis with O 2 and NO 2 - microsensors showed that the thickness of the activity zones varied as a function of bulk O 2 and NO 2 - concentrations and flow rate.