养殖氧化塘周边地下水氨氧化细菌分布及环境因子的影响

为了研究畜禽养殖场的废水氧化塘对周边地下水的影响,我们通过在海盐县某养猪场氧化塘周边区域布井,采集相应点位的地下水,并采用分子生物学手段实时荧光定量核酸扩增检测（real-time quantitative PCR detecting system,qPCR）和水质理化性质分析手段,研究氧化塘周边相应位点地下水中的氨氧化细菌（ammoniaoxidizing bacteria,AOB）的丰度和水质情况,并结合相关系数,探究AOB丰度与环境因子之间的关系。结果表明：在相应位点的地下水中AOB的丰度与相应地下水中氨氮、硝氮和总氮的质量浓度呈显著正相关,特别是AOB丰度与硝氮呈极显著正相关关系（P〈...

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Published in浙江大学学报（农业与生命科学版） Vol. 42; no. 5; pp. 589 - 597
Main Author 任娟 张磊 曾令藻 吴劳生 施加春
Format Journal Article
LanguageChinese
Published 浙江大学环境与资源学院土水资源与环境研究所,杭州,310058 2016
Subjects
Pearson相关系数
地下水
氨氧化细菌（AOB）
环境因子
Pearson相关系数
ammonia-oxidizing bacteria (AOB)
environmental factors
地下水
环境因子
groundwater
氨氧化细菌(AOB)
Pearson correlation coefficient
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Summary:为了研究畜禽养殖场的废水氧化塘对周边地下水的影响,我们通过在海盐县某养猪场氧化塘周边区域布井,采集相应点位的地下水,并采用分子生物学手段实时荧光定量核酸扩增检测（real-time quantitative PCR detecting system,qPCR）和水质理化性质分析手段,研究氧化塘周边相应位点地下水中的氨氧化细菌（ammoniaoxidizing bacteria,AOB）的丰度和水质情况,并结合相关系数,探究AOB丰度与环境因子之间的关系。结果表明：在相应位点的地下水中AOB的丰度与相应地下水中氨氮、硝氮和总氮的质量浓度呈显著正相关,特别是AOB丰度与硝氮呈极显著正相关关系（P〈0.01）;同时也发现AOB丰度受pH影响,当pH〉7.8时,AOB丰度较大,但AOB丰度和pH线性相关性不显著。
Bibliography:33-1247/S
groundwater; ammonia-oxidizing bacteria（AOB）; environmental factors; Pearson correlation coefficient
Groundwater is a precious natural resource,which is used as potable water,industrial water and agricultural water.With the development of breeding industry,lots of waste water and solid wastes were discharged to the environment,which has caused serious groundwater safety problems,especially high nitrogen concentration.Groundwater is a kind of comparatively well living environment for micro-organisms.Because it has abundant nutrition,water,adaptive temperature and pH and all these conditions are beneficial for growth and reproduction of micro-organisams.In water nitrate cycle,micro-organisms play a significant role.The first and rate limiting step of nitrification is ammoxidation,and the functional gene is ammonia monooxygenase,which existed in ammoniaoxidizing bacteria and ammonia-oxidizing archaea.However,the factors affecting ammonia-oxidizing bacteria distribution pattern in groundwater and the infl
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2015.11.182