Production of cyclosporine a by submerged fermentation from a local isolate of Penicillium fellutanum

Six locally isolated strains of Penicillium were checked for their potential to produce cyclosporine A through submerged fermentation. The medium used for drug production was composed of glucose, 5%; peptone, 1%; KH 2 PO 4 , 0.5% and KCl, 0.25% (w/v). Butyl acetate was used to extract the fermentati...

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Published inIndian journal of pharmaceutical sciences Vol. 74; no. 4; pp. 372 - 374
Main Authors Anjum, Tehmina, Azam, Asma, Irum, Wajiha
Format Journal Article
LanguageEnglish
Published India Medknow Publications 01.07.2012
Medknow Publications and Media Pvt. Ltd
Medknow Publications & Media Pvt. Ltd
Medknow Publications & Media Pvt Ltd
Subjects
Biomass
Chemical properties
Chromatography
Cyclosporine
Drugs
Fermentation
Fungi
Identification and classification
Microbiology
Penicillium
Penicillium fellutanum
Product/Service Evaluations
Short Communication
India
Cyclosporine A
Penicillium
high performance liquid chromatography
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Summary:Six locally isolated strains of Penicillium were checked for their potential to produce cyclosporine A through submerged fermentation. The medium used for drug production was composed of glucose, 5%; peptone, 1%; KH 2 PO 4 , 0.5% and KCl, 0.25% (w/v). Butyl acetate was used to extract the fermentation medium for cyclosporine ′A′ analysis. The confirmation analysis was done through high performance liquid chromatography and the chromatograms obtained were compared with that of Sandimmun Neoral® capsule (Novartis) containing 100 mg of cyclosporine and with the external standard cyclosporine A 98.5% pure. Only chromatogram of Penicillium fellutanum (FCBP 937) isolated from Guava fruit showed a peak at 2.768, which was comparable with both the standards. The amount of drug calculated was 16.18 μg/ml.
Bibliography:ObjectType-Article-1
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ISSN:0250-474X
1998-3743
DOI:10.4103/0250-474x.107082