Influenza vaccine: Development of a novel intranasal and subcutaneous recombinant adjuvant

• Published in: Vaccine Vol. 31; no. 37; pp. 4009 - 4016
• Main Authors: Segura-Velázquez, R, Cervantes, J, Acosta, E, Sánchez-Betancourt, I, Villalobos, N, Rodarte, L.F, Restelli, M, Fragoso, G, Sciutto, E
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 20.08.2013; Elsevier; Elsevier Limited
• Subjects: Adjuvant; Adjuvants; Adjuvants, Immunologic - genetics; Adjuvants, Immunologic - pharmacology; Administration, Intranasal; Allergy and Immunology; Animals; antibodies; Antibodies, Viral - blood; Applied microbiology; Bacteriophage M13 - genetics; Biological and medical sciences; blood serum; Bronchoalveolar Lavage Fluid - immunology; Female; Fundamental and applied biological sciences. Psychology; Hogs; human influenza; Immunization; immunoglobulin A; Immunoglobulin A - blood; immunoglobulin G; Immunoglobulin G - blood; Influenza; Influenza Vaccines - administration & dosage; Influenza Vaccines - immunology; Influenza Vaccines - pharmacology; Infusions, Parenteral; Injections, Subcutaneous; Intranasal immunization; Maternal Age; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Peptides; Peptides - immunology; Phages; Polysorbates - administration & dosage; Polysorbates - pharmacology; Squalene - administration & dosage; Squalene - pharmacology; Swine; synthetic peptides; Taenia; Taenia crassiceps; Vaccination; Vaccination - methods; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Virology; Mexico; Argentina; Intranasal immunization; Adjuvant; Phages; Influenza; Vaccination; Immunization; Vaccine; Intranasal administration; Infection; Virus; Viral disease; Immunological adjuvant; Influenza A; Phage
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2013.05.044

Highlights • GK-1 was recombinantly expressed fused to the pVIII surface protein of the paghemid M13 (FGK1). • Intranasal and parenteral co-immunization of FGK1 with the influenza vaccine increase specific levels of antibodies in mice. • Parenteral co-immunization of FGK1 with the influenza vaccine increases levels of specific IgG in pigs. • A new low costly adjuvant, adequate for veterinary purposes is developed.