Expression of the aging gene p66Shc is increased in peripheral blood monocytes of patients with acute coronary syndrome but not with stable coronary artery disease

• Published in: Atherosclerosis Vol. 220; no. 1; pp. 282 - 286
• Main Authors: Franzeck, Fabian C., Hof, Danielle, Spescha, Remo D., Hasun, Matthias, Akhmedov, Alexander, Steffel, Jan, Shi, Yi, Cosentino, Francesco, Tanner, Felix C., von Eckardstein, Arnold, Maier, Willibald, Lüscher, Thomas F., Wyss, Christophe A., Camici, Giovanni G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ireland Ltd 01.01.2012; Elsevier
• Subjects: Acute Coronary Syndrome; Acute Coronary Syndrome - blood; Acute Coronary Syndrome - diagnostic imaging; Acute Coronary Syndrome - genetics; animal models; atherogenesis; atherosclerosis; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; biomarkers; blood; Blood and lymphatic vessels; C-reactive protein; Cardiology. Vascular system; Cardiovascular; chemistry; Chi-Square Distribution; Coronary Angiography; Coronary artery disease; Coronary Artery Disease - blood; Coronary Artery Disease - diagnostic imaging; Coronary Artery Disease - genetics; Coronary heart disease; coronary vessels; correlation; genes; genetics; Heart; Humans; inflammation; Lipid Peroxidation; Lipid Peroxidation - genetics; Male; malondialdehyde; Malondialdehyde - blood; Medical sciences; Middle Aged; monocytes; Monocytes - chemistry; myocardial infarction; Oxidative stress; Oxidative Stress - genetics; p66shc; pathogenesis; patients; quantitative polymerase chain reaction; radiography; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger; RNA, Messenger - blood; Shc Signaling Adaptor Proteins; Shc Signaling Adaptor Proteins - genetics; Src Homology 2 Domain-Containing, Transforming Protein 1; Switzerland; Up-Regulation; Switzerland; Oxidative stress; p66shc; Acute coronary syndrome; Coronary artery disease; Human; Senescence; Monocyte; Cardiovascular disease; Gene expression; Myocardial disease; Coronary heart disease; Vascular disease; Atherosclerosis
• ISSN: 0021-9150; 1879-1484; 1879-1484
• DOI: 10.1016/j.atherosclerosis.2011.10.035

The interplay between oxidative stress and inflammation is crucial in the pathogenesis of atherosclerosis. The adaptor protein p66Shc is implicated in atherogenesis and oxidative stress related responses in animal models of diseases. However, its role in humans remains to be defined. In this study, we hypothesized that expression of p66Shc increases in peripheral blood monocytes of patients affected by acute coronary syndromes (ACS). Male subjects aged 59±4 (mean±SD) years admitted for cardiac catheterization were subdivided in three groups: (a) no local stenosis for the control group, (b) at least one stenosis ≥75% in either left, circumflex or right coronary artery for the coronary artery disease (CAD) group or (c) ST-elevation/non-ST-elevation myocardial infarction for the ACS group. Monocytes were isolated from whole blood and p66Shc RNA levels were determined by quantitative real time PCR. p66Shc RNA levels were increased in ACS patients as compared to CAD (p=0.007) and controls (p=0.0249). Furthermore, malondialdehyde (MDA) and C-reactive protein (CRP) were increased in plasma of ACS patients. Levels of MDA correlated positively to p66Shc (r=0.376, p=0.01). Our data demonstrate increased p66Shc levels in monocytes of ACS but not CAD patients. This study suggests an involvement of p66Shc in the transition of a stable CAD to an ACS patient. p66Shc was associated with states of increased oxidative stress. Further work is needed to understand whether p66Shc may represent a possible pharmacological target or whether it represents an interesting novel biomarker.