Tyrosine phosphorylation of GluR2 is required for insulin-stimulated AMPA receptor endocytosis and LTD

• Published in: The EMBO journal Vol. 23; no. 5; pp. 1040 - 1050
• Main Authors: Ahmadian, Gholamreza, Ju, William, Liu, Lidong, Wyszynski, Michael, Lee, Sang Hyoung, Dunah, Anthone W, Taghibiglou, Changiz, Wang, Yushan, Lu, Jie, Wong, Tak Pan, Sheng, Morgan, Wang, Yu Tian
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 10.03.2004; Nature Publishing Group UK; Springer Nature B.V; Nature Publishing Group
• Subjects: Aging - physiology; Amino Acid Sequence; Amino acids; AMPA receptor; Animals; Cells, Cultured; EMBO20; EMBO27; endocytosis; Endocytosis - drug effects; hippocampal slices; Hippocampus - cytology; Hippocampus - metabolism; Humans; In Vitro Techniques; Insulin; Insulin - pharmacology; Long-Term Synaptic Depression; LTP; Molecular Sequence Data; Mutation; Mutation - genetics; Neurons - metabolism; Phosphorylation; Phosphotyrosine - metabolism; Plasticity; Protein Subunits - metabolism; Rats; Rats, Sprague-Dawley; Receptors, AMPA - chemistry; Receptors, AMPA - genetics; Receptors, AMPA - metabolism; Sequence Alignment; Synaptic Transmission; Time Factors; tyrosine phosphorylation; endocytosis; LTP; AMPA receptor; tyrosine phosphorylation; hippocampal slices
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1038/sj.emboj.7600126

The α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA) subtype of glutamate receptors is subject to functionally distinct constitutive and regulated clathrin‐dependent endocytosis, contributing to various forms of synaptic plasticity. In HEK293 cells transiently expressing GluR1 or GluR2 mutants containing domain deletions or point mutations in their intracellular carboxyl termini (CT), we found that deletion of the first 10 amino acids (834–843) selectively reduced the rate of constitutive AMPA receptor endocytosis, whereas truncation of the last 15 amino acids of the GluR2 CT, or point mutation of the tyrosine residues in this region, only eliminated the regulated (insulin‐stimulated) endocytosis. Moreover, in hippocampal slices, both insulin treatment and low‐frequency stimulation (LFS) specifically stimulated tyrosine phosphorylation of the GluR2 subunits of native AMPA receptors, and the enhanced phosphorylation appears necessary for both insulin‐ and LFS‐induced long‐term depression of AMPA receptor‐mediated excitatory postsynaptic currents. Thus, our results demonstrate that constitutive and regulated AMPA receptor endocytosis requires different sequences within GluR CTs and tyrosine phosphorylation of GluR2 CT is required for the regulated AMPA receptor endocytosis and hence the expression of certain forms of synaptic plasticity.