Low-temperature biodegradation of aniline by freely suspended and magnetic modified Pseudomonas migulae AN-1

• Published in: Applied microbiology and biotechnology Vol. 99; no. 12; pp. 5317 - 5326
• Main Authors: Liu, Yong-Bo, Qu, Dan, Wen, Yu-Juan, Ren, He-Jun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2015; Springer; Springer Nature B.V
• Subjects: Analysis; Aniline; Aniline Compounds - metabolism; Aquifers; Assembling; Bacteria; Biochemistry; Biodegradation; Biodegradation, Environmental; Biomedical and Life Sciences; Bioremediation; Biotechnology; Cold; Cold Temperature; Degradation; Environmental Biotechnology; Environmental perception; Evolutionary; Groundwater; Groundwater - microbiology; Life Sciences; Low temperature; Magnetics; Microbial Genetics and Genomics; Microbiology; Nanoparticles; Physiological aspects; Pollutants; Pseudomonas; Pseudomonas - chemistry; Pseudomonas - genetics; Pseudomonas - growth & development; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Pseudomonas migulae; Soil Pollutants - metabolism; Strain; Studies; Temperature; Water Pollutants, Chemical - metabolism; Water treatment; China; Biodegradation; nanoparticles; Aniline; Low temperature; AN-1; Fe; O
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-015-6399-2

Aniline is of great environmental concern with regards to widespread occurrence in water and soil and increasing threat into the life forms. Bioremediation involving the use of degrading bacterium in the removal of aniline is the most promising process, yet inhibited under low temperature usually. In the present study, a new psychrotrophic bacterial strain isolated from groundwater, designated AN-1, was shown to be capable of aniline degradation in a concentration range of 135–2202 mg L −1 within 72 h at 10 °C. Strain AN-1 was proposed to be a Pseudomonas migulae group of bacteria based on the evolutionary relationship and the morphological and biochemical characteristics. The pH, NaH 2 PO 4 , and aniline concentration were used as independent variables to optimize the aniline removal by AN-1 at 10 °C, and a statistically significant ( R 2  = 0.9230, p  < 0.005) quadratic polynomial mathematical model was suggested. Moreover, an efficient biocomposite by assembling Fe 3 O 4 nanoparticles onto the surface of AN-1 cells was constructed. Compared with free cells, the microbial cell/Fe 3 O 4 biocomposite had the same biodegradation activity but exhibited remarkable reusability. This study highlights AN-1 might be a promising candidate for aniline removal from wastewater at low temperatures.