Transcriptional control of floral anthocyanin pigmentation in monkeyflowers (Mimulus)

• Published in: The New phytologist Vol. 204; no. 4; pp. 1013 - 1027
• Main Authors: Yuan, Yao‐Wu, Sagawa, Janelle M., Frost, Laura, Vela, James P., Bradshaw, Harvey D.
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.12.2014; Wiley Subscription Services, Inc
• Subjects: anthocyanin pigmentation; Anthocyanins; Anthocyanins - genetics; Anthocyanins - metabolism; autoregulation; Color; Colour; Corolla; Evolution & development; flower color; Flowers; Flowers - genetics; Flowers - metabolism; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genomics; Mimulus; Mimulus - genetics; Mimulus - metabolism; Model testing; monkeyflowers (Mimulus); Mutants; Mutation; MYB‐bHLH‐WD40; natural variation; Nectar; Petals; phenotypic evolution; Phylogeny; Pigmentation; Pigments, Biological - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plants, Genetically Modified; Pollinators; Reverse transcriptase polymerase chain reaction; Sequencing; Speciation; Transcription; Transcriptomes; phenotypic evolution; flower color; anthocyanin pigmentation; MYB-bHLH-WD40; natural variation; autoregulation; monkeyflowers (Mimulus)
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.12968

A molecular description of the control of floral pigmentation in a multi-species group displaying various flower color patterns is of great interest for understanding the molecular bases of phenotypic diversification and pollinator-mediated speciation. Through transcriptome profiling, mutant analyses and transgenic experiments, we aim to establish a ‘baseline’ floral anthocyanin regulation model in Mimulus lewisii and to examine the different ways of tinkering with this model in generating the diversity of floral anthocyanin patterns in other Mimulus species. We find one WD40 and one bHLH gene controlling anthocyanin pigmentation in the entire corolla of M. lewisii and two R2R3-MYB genes, PELAN and NEGAN, controlling anthocyanin production in the petal lobe and nectar guide, respectively. The autoregulation of NEGAN might be a critical property to generate anthocyanin spots. Independent losses of PELAN expression (via different mechanisms) explain two natural yellow-flowered populations of M. cardinalis (typically red-flowered). The NEGAN ortholog is the only anthocyanin-activating MYB expressed in the M. guttatus flowers. The mutant lines and transgenic tools available for M. lewisii will enable gene-by-gene replacement experiments to dissect the genetic and developmental bases of more complex floral color patterns, and to test hypotheses on phenotypic evolution in general.