Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration

•Mesenchymal stem cell exosomes promoted myogenesis and angiogenesis in vitro.•Mesenchymal stem cell exosomes accelerated muscle regeneration in a mouse injury model.•Mesenchymal stem cell exosomes had low concentrations of repair-related cytokines.•Many repair-related microRNAs were identified in m...

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Published inFEBS letters Vol. 589; no. 11; pp. 1257 - 1265
Main Authors Nakamura, Yoshihiro, Miyaki, Shigeru, Ishitobi, Hiroyuki, Matsuyama, Sho, Nakasa, Tomoyuki, Kamei, Naosuke, Akimoto, Takayuki, Higashi, Yukihito, Ochi, Mitsuo
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 08.05.2015
Subjects
angiogenesis
Animals
Cell Line
cytokines
Exosome
Exosomes
Humans
Mesenchymal stem cell
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mice
MicroRNA
MicroRNAs - biosynthesis
Muscle Development
Muscle, Skeletal - cytology
Muscle, Skeletal - physiology
muscles
Myoblasts, Skeletal - cytology
Myoblasts, Skeletal - metabolism
Regeneration
Regeneration - physiology
Skeletal muscle
stem cells
tissue repair
Mesenchymal stem cell
Regeneration
MicroRNA
Exosome
Skeletal muscle
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Summary:•Mesenchymal stem cell exosomes promoted myogenesis and angiogenesis in vitro.•Mesenchymal stem cell exosomes accelerated muscle regeneration in a mouse injury model.•Mesenchymal stem cell exosomes had low concentrations of repair-related cytokines.•Many repair-related microRNAs were identified in mesenchymal stem cell exosomes.•MicroRNA-494 in mesenchymal stem cell exosomes enhanced myogenesis and angiogenesis. Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regeneration in an in vivo model of muscle injury. Although MSC exosomes had low concentrations of muscle-repair-related cytokines, a number of repair-related miRNAs were identified. This study suggests that the MSC-derived exosomes promote muscle regeneration by enhancing myogenesis and angiogenesis, which is at least in part mediated by miRNAs such as miR-494.
Bibliography:Y.N.: the conception and design of the study, acquisition of data, analysis and interpretation of data, manuscript writing; S.M.: the conception and design of the study, acquisition of data, analysis and interpretation of data, manuscript writing; H.I.: acquisition of data, analysis and interpretation of data; S.M.: acquisition of data; T.N.: analysis and interpretation of data; N.K.; analysis and interpretation of data; T.A.: acquisition of data, analysis and interpretation of data; Y.H.: analysis and interpretation of data; M.O.: final approval of the version to be submitted.
Yoshihiro Nakamura and Shigeru Miyaki contributed equally to this work.
Author contributions
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SourceType-Scholarly Journals-1
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content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2015.03.031