ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

• Published in: Nature methods Vol. 12; no. 10; pp. 963 - 965
• Main Authors: Schmidl, Christian, Rendeiro, André F, Sheffield, Nathan C, Bock, Christoph
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.10.2015; Nature Publishing Group
• Subjects: 38/1; 38/23; 45/15; 631/1647/2217/2088; 631/208/177; 631/208/191; 631/337/100; Binding sites; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical Engineering/Biotechnology; brief-communication; Chromatin; Chromatin Immunoprecipitation - economics; Chromatin Immunoprecipitation - instrumentation; Chromatin Immunoprecipitation - methods; Epigenetics; Genome, Human; Genomes; Histones; Histones - metabolism; Humans; Identification and classification; Immunoassay; Innovations; K562 Cells; Life Sciences; Proteomics; Transcription factors; Transcription Factors - analysis; Transcription Factors - metabolism
• ISSN: 1548-7091; 1548-7105
• DOI: 10.1038/nmeth.3542

ChIPmentation combines chromatin immunoprecipitation with on-bead tagmentation for rapid and highly robust ChIP-seq library preparation. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.