Alteration of Gene Expressions by the Overexpression of Mitochondrial Phospholipid Hydroperoxide Glutathione Peroxidase (mtPHGPx)

• Published in: Gene expression Vol. 11; no. 2; pp. 77 - 83
• Main Authors: KITAHARA, JUN, CHIBA, NOBUYOSHI, SAKAMOTO, HIKARU, NAKAGAWA, YASUHITO
• Format: Journal Article
• Language: English
• Published: Elmsford, NY Cognizant Communication Corporation 01.01.2003; Xia & He Publishing
• Subjects: Animals; Antioxidants - pharmacology; Basophilic leukemia; Cloning; Cytokines; Cytoskeletal Proteins - genetics; Cytoskeleton; Differential display; Down-Regulation; Enzymes; Gene expression; Gene Expression - drug effects; Gene Expression Profiling; Genes; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Growth Differentiation Factor 1; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Intercellular Signaling Peptides and Proteins - genetics; Leukemia; Metabolism; Mitochondria; Mitochondria - enzymology; Molecular weight; Myosin; Nerve Tissue Proteins - genetics; Peroxidase; Phospholipid Hydroperoxide Glutathione Peroxidase (phgpx) Mitochondria Reactive Oxygen Species (ros) Differential Display; Phospholipids; Proteins; Rats; Reactive Oxygen Species; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal transduction; Steroids; Thiocarbamates - pharmacology; Trace levels; Tubulin; Tumor Cells, Cultured; Up-Regulation; Vimentin; United Kingdom > UK; Germany
• ISSN: 1052-2166; 1555-3884
• DOI: 10.3727/000000003108748973

To determine the effect on gene expression of trace levels of reactive oxygen species from mitochondria, we used the mRNA differential display technique to compare gene expression in two cell lines: M15, which overexpresses mitochondrial phospholipid hydroperoxide glutathione peroxidase (mtPHGPx), in rat basophilic leukemia RBL-2H3 cells, and a control cell line, S1. We isolated 27 differentially expressed genes, including 10 previously unreported sequences. These genes included cytoskeletal proteins (β-tubulin, nonmuscle myosin alkali light chain, and vimentin), growth or proliferation regulators [growth differentiation factor 1 (Gdf-1), Rap1a, and inhibitor of growth 3 (Ing3)], and others. Although the expression of most of the isolated genes did not respond to ROS (hydrogen peroxide) or antioxidant (pyrolidine dithiocarbamate) treatment, the expression of Gdf-1 was downregulated by hydrogen peroxide treatment. Thus, low levels of ROS produced in mitochondria during normal cellular metabolism can modulate gene expression.