2种色型黄粉虫酚氧化酶同工酶的比较
为黄粉虫（Tenebrio molitor L.）品种选育、体色分化及免疫防御研究提供参考,采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法,对黄、黑2种色型黄粉虫各发育期的酚氧化酶（包括单酚氧化酶和二酚氧化酶）同工酶酶谱及酶活性进行比较研究。结果表明：在2种色型黄粉虫的各发育期,共发现迁移率不同的单酚氧化酶（MPO）同工酶酶带6条、二酚氧化酶（DPO）同工酶酶带5条,其中MPO酶带分布在3个泳动区,DPO酶带分布在2个泳动区,黑色型卵比黄色型卵多1条MPO特征性酶带;并且黑色型卵、幼虫和成虫的MPO和DPO活性显著高于黄色型卵、幼虫和成虫;但在蛹期,MPO活性黄色型比黑色型高,而DPO活性...
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| Published in | 浙江大学学报（农业与生命科学版） Vol. 38; no. 4; pp. 377 - 386 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
四川农业大学 森林保护学省级重点实验室 ,四川 雅安 625014%四川农业大学 长江上游林业生态工程四川省重点实验室 ,四川 雅安 625014%四川农业大学 经济管理学院 ,四川 成都 611130%四川农业大学动物科技学院 ,四川 雅安 625014%四川农业大学 生命科学与理学院 生物系 ,四川 雅安 625014
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-9209 |
| DOI | 10.3785/j.issn.1008‐9209.2012.04.004 |
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| Summary: | 为黄粉虫（Tenebrio molitor L.）品种选育、体色分化及免疫防御研究提供参考,采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法,对黄、黑2种色型黄粉虫各发育期的酚氧化酶（包括单酚氧化酶和二酚氧化酶）同工酶酶谱及酶活性进行比较研究。结果表明：在2种色型黄粉虫的各发育期,共发现迁移率不同的单酚氧化酶（MPO）同工酶酶带6条、二酚氧化酶（DPO）同工酶酶带5条,其中MPO酶带分布在3个泳动区,DPO酶带分布在2个泳动区,黑色型卵比黄色型卵多1条MPO特征性酶带;并且黑色型卵、幼虫和成虫的MPO和DPO活性显著高于黄色型卵、幼虫和成虫;但在蛹期,MPO活性黄色型比黑色型高,而DPO活性则是黑色型比黄色型高;此外,同一色型黄粉虫在不同发育阶段的MPO和DPO同工酶酶带数、酶活性强弱及迁移率变化明显,其中,卵期有MPO和DPO特有酶带各1条,幼虫期只有1条DPO特有酶带;同时,各发育阶段的MPO和DPO同工酶酶谱及酶活性变化规律不同,由卵至成虫期,MPO活性变化趋势为：卵期〉蛹期〉成虫期〉幼虫期,DPO活性变化趋势为：幼虫期〉成虫期〉蛹期〉卵期。上述结果为进一步研究黄粉虫的体色分化、免疫防御和品种选育奠定了理论基础。 |
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| Bibliography: | In order to provide some references for studying the body-color diversity, immune defence and variety selection of Tenebrio rnolitor, the phenoloxidase （including monophenol oxidase and diphenol oxidase） isozyme zymograms and activity at different developmental stages of the yellow- and black-color varieties of T. molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry. The results showed that, during their developmental stages, six monophenol oxidase （MPO） bands and five diphenol oxidase （DPO） bands with different mobility were found, in which the MPO bands were distributed in three zones, while the DPO bands were in two zones. Meanwhile, the eggs of the black-color variety showed one more distinctive MPO bands compared with those of the yellow-color variety. Moreover, the MPO and DPO activities of black-color variety eggs, larvae and adults were significantly higher than those of the yellow-color ones. But at the pupal stage, the MPO activity of the yellow-color variety |
| ISSN: | 1008-9209 |
| DOI: | 10.3785/j.issn.1008‐9209.2012.04.004 |