在低盐度胁迫下鲻幼鱼鳃丝 Na^＋-K^＋-ATP 酶活性及其β亚基 mRNA 表达与显微结构变化

探讨在低盐度胁迫下鲻（Mugil cephalus）幼鱼鳃丝 Na＋-K＋-ATP 酶活性、Na＋-K＋-ATP 酶β亚基 mRNA表达量变化以及对鳃丝组织显微结构的影响。结果表明：在盐度分别为20（对照组），15，10，5和0实验条件下，20 d 后各低盐度组鳃丝 Na＋-K＋-ATP 酶活性均高于对照组；0和5盐度组鳃丝 Na＋-K＋-ATP 酶活性在实验过程中表现为持续上升的趋势，前期升高幅度大，后期升高幅度减小；10和15盐度组鳃丝 Na＋-K＋-ATP 酶活性在15 d后达到峰值，至20 d 呈现小幅下降趋势。鳃丝 Na＋-K＋-ATP 酶β亚基 mRNA 表达的变化趋势与 Na＋-...

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Published in浙江大学学报（农业与生命科学版） Vol. 40; no. 2; pp. 223 - 230
Main Author 李磊 蒋玫 王云龙 吴庆元 牛俊翔 沈新强
Format Journal Article
LanguageChinese
Published 中国水产科学研究院东海水产研究所,上海,200090%上海海洋大学海洋科学学院,上海,201306 2014
Subjects
Na^＋-K^＋-ATP
显微结构
盐度
酶
鲻幼鱼
鳃丝
显微结构
salinity
gill filament
鲻幼鱼
Na+-K+-ATP 酶β亚基 mRNA
Na+-K+-ATP 酶
盐度
Na+-K+-ATPase
Na+-K+-ATPase β-subunit mRNA
鳃丝
juvenile Mugil cephalus
microscopical structure
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2013.09.031

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Summary:探讨在低盐度胁迫下鲻（Mugil cephalus）幼鱼鳃丝 Na＋-K＋-ATP 酶活性、Na＋-K＋-ATP 酶β亚基 mRNA表达量变化以及对鳃丝组织显微结构的影响。结果表明：在盐度分别为20（对照组），15，10，5和0实验条件下，20 d 后各低盐度组鳃丝 Na＋-K＋-ATP 酶活性均高于对照组；0和5盐度组鳃丝 Na＋-K＋-ATP 酶活性在实验过程中表现为持续上升的趋势，前期升高幅度大，后期升高幅度减小；10和15盐度组鳃丝 Na＋-K＋-ATP 酶活性在15 d后达到峰值，至20 d 呈现小幅下降趋势。鳃丝 Na＋-K＋-ATP 酶β亚基 mRNA 表达的变化趋势与 Na＋-K＋-ATP酶活性的变化趋势基本保持同步，显示出了其对 Na＋-K＋-ATP 酶活性的调控作用。对鳃丝显微结构观察的结果显示，随着盐度的降低，鲻幼鱼鳃小片逐渐变宽，伴随间距缩小，同时泌氯细胞数量逐渐减少，体积缩小，扁平细胞体积增大，黏液细胞数量增多。这表明在低盐度胁迫下鲻幼鱼鳃的生理功能和组织结构具有随外界水体盐度变化的适应能力。
Bibliography:Na＋-K＋-ATP 酶β亚基 mRNA;juvenile Mugil cephalus;salinity;gill filament;Na＋-K＋-ATPase;Na＋-K＋-ATPase β-subunit mRNA;microscopical structure
Summary In natural aquatic system,salinity can largely influence growth,breeding,and even fishes survival. In general, euryhaline fishes often possess strong osmotic adjustment capacity. The gill filaments play an important role in the maintenance of blood ion and acid-base balance in seawater-acclimated fishes,especially if there is a sudden change in water salinity.Seawater-acclimated fishes drink seawater to counter osmotic loss of water,and secrete excess Na＋ and Cl- across the gill filaments.When there is a change between freshwater and seawater,euryhaline fishes must transform their gill filaments from an ion-absorbing epithelium to an ion-secreting epithelium,or vice versa.Associated with this transformation is change of Na＋/K＋-ATPase activity,Na＋/K＋-ATPase gene expression and structural change of the gill.Euryhaline teleost fishes,such as killifish Fundulus heteroclitus
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2013.09.031