黄瓜白粉病抗性序列相关扩增多态性的分子标记研究

以高抗和高感白粉病的黄瓜亲本及其杂交后代F2分离群体为材料,应用序列相关扩增多态性（sequence-related amplified polymorphism,SRAP）技术开展与黄瓜白粉病抗性基因连锁的SRAP分子标记研究.结果表明：从437对SRAP引物组合中筛选出62对能够在2个亲本抗、感池间扩增出稳定多态性条带的引物组合,进一步用这些引物分析F2群体,发现有1对引物组合Me1/Em9扩增出的SRAP标记（Me1/Em9—284 bp）能与黄瓜抗白粉病基因连锁,遗传距离为9.8 c M.获得的分子标记对克隆抗病基因和利用分子标记辅助选择提高育种效率具有重要意义....

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Published in浙江大学学报(农业与生命科学版) Vol. 37; no. 4; pp. 387 - 392
Main Author 景然 陈新娟 朱育强 张鹏 张玉琼 周胜军
Format Journal Article
LanguageChinese
Published 安徽农业大学生命科学学院,安徽合肥,230036%浙江省农业科学院蔬菜研究所,浙江杭州,310021 2011
Subjects
序列相关扩增多态性分子标记
白粉病
黄瓜
白粉病
序列相关扩增多态性分子标记
黄瓜
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2011.04.005

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Summary:以高抗和高感白粉病的黄瓜亲本及其杂交后代F2分离群体为材料,应用序列相关扩增多态性（sequence-related amplified polymorphism,SRAP）技术开展与黄瓜白粉病抗性基因连锁的SRAP分子标记研究.结果表明：从437对SRAP引物组合中筛选出62对能够在2个亲本抗、感池间扩增出稳定多态性条带的引物组合,进一步用这些引物分析F2群体,发现有1对引物组合Me1/Em9扩增出的SRAP标记（Me1/Em9—284 bp）能与黄瓜抗白粉病基因连锁,遗传距离为9.8 c M.获得的分子标记对克隆抗病基因和利用分子标记辅助选择提高育种效率具有重要意义.
Bibliography:cucumber; powdery mildew; sequence-related amplified polymorphism（SRAP） marker
JING Ran,CHEN Xin-juan,ZHU Yu-qiang,ZHANG Peng,ZHANG Yu-qiong,ZHOU Sheng-jun（1.School of Life Science,Anhui Agricultural University,Hefei 230036,China;2.Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China）
33-1247/S
With a F2 population between a resistant parent and susceptible parent,SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber.Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from 437 pairs of SRAP primer combinations.Further analyzing the F2 segregating populations using these primers,one SRAP marker of Me1/Em9—284 bp linked to the powdery mildew resistance was found,with 9.8 cM genetic distance.The marker can be used for cloning disease-resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection.
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2011.04.005