The Minor Histocompatibility Antigen HA-1: A Diallelic Gene with a Single Amino Acid Polymorphism

• Published in: Science (American Association for the Advancement of Science) Vol. 279; no. 5353; pp. 1054 - 1057
• Main Authors: Joke M. M. den Haan, Meadows, Leslie M., Wang, Wei, Pool, Jos, Blokland, Els, Bishop, Tracie L., Reinhardus, Carla, Shabanowitz, Jeffrey, Offringa, Rienk, Hunt, Donald F., Engelhard, Victor H., Goulmy, Els
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 13.02.1998; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Alleles; Amino Acid Sequence; Amino acids; Analysis; Antigens; Biological and medical sciences; Bone marrow; Bone Marrow Transplantation - adverse effects; Cell Line; Cell Line, Transformed; Cell lines; Complementary DNA; DNA; Family (Sociological Unit); Female; Fractions; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genes; Genetics of the immune response; Graft vs Host Disease - immunology; HeLa cells; Hematology; Histocompatibility Testing; HLA-A Antigens - immunology; Humans; Immunobiology; Immunology; Leukocytes; Male; Mass Spectrometry; Mass spectroscopy; Minor Histocompatibility Antigens - chemistry; Minor Histocompatibility Antigens - genetics; Minor Histocompatibility Antigens - immunology; Minor Histocompatibility Loci; Molecules; Oligopeptides - chemistry; Oligopeptides - genetics; Oligopeptides - immunology; Phenotype; Polymerase Chain Reaction; Polymorphism, Genetic; T lymphocytes; T-Lymphocytes, Cytotoxic - immunology; Transplants & implants; Human; HLA-System; Typing; Major histocompatibility system; Graft versus host reaction; Minor histocompatibility system; Transplantation; Immunogenetics; Gene; Bone marrow; Genetics; Graft; Polymorphism
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.279.5353.1054

The minor histocompatibility antigen (mHag) HA-1 is the only known mHag for which mismatching is correlated with the development of severe graft versus host disease (GvHD) after human leukocyte antigen-identical bone marrow transplantation. HA-1 was found to be a nonapeptide derived from an allele of the KIAA0223 gene. The HA-1-negative allelic counterpart encoded by KIAA0223 had one amino acid difference from HA-1. Family analysis with HA-1 allele-specific polymerase chain reaction showed an exact correlation between this allelic polymorphism and the HA-1 phenotype. HA-1 allele typing of donor and recipient should improve donor selection and allow the determination of bone marrow transplantation recipients with high risk for HA-1-induced GvHD development.