食品中牛奶过敏原成分LAMP检测方法的建立
目的：建立食品过敏原牛奶成分LAMP(loop-mediated isothermal amplification)检测方法，并与实时荧光PCR(real-time PCR)检测方法比对。方法针对牛线粒体细胞色素b(cyt-b)基因设计LAMP引物并建立反应体系，在特异性和灵敏度方面与real-time PCR检测方法比对。结果本研究建立的LAMP方法检测9份不同品牌的牛奶和羊奶及其加工制品，没有出现交叉反应，具有良好的特异性。该方法的检测灵敏度为0.5%，与real-time PCR方法检测灵敏度相当。检测了69份实际样品，检测结果与real-time PCR检测结果一致。结论本研究建立的食...
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| Published in | 食品安全质量检测学报 no. 4; pp. 1039 - 1044 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
北京农学院，北京 102206%北京出入境检验检疫局,北京,100026%北京农学院,北京,102206
2014
北京出入境检验检疫局，北京 100026 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的：建立食品过敏原牛奶成分LAMP(loop-mediated isothermal amplification)检测方法，并与实时荧光PCR(real-time PCR)检测方法比对。方法针对牛线粒体细胞色素b(cyt-b)基因设计LAMP引物并建立反应体系，在特异性和灵敏度方面与real-time PCR检测方法比对。结果本研究建立的LAMP方法检测9份不同品牌的牛奶和羊奶及其加工制品，没有出现交叉反应，具有良好的特异性。该方法的检测灵敏度为0.5%，与real-time PCR方法检测灵敏度相当。检测了69份实际样品，检测结果与real-time PCR检测结果一致。结论本研究建立的食品过敏原牛奶成分LAMP检测方法简单经济，检测结果可靠，可有效缩短检测时间，适用于过敏原牛奶成分的检测，具有良好的应用前景。 |
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| AbstractList | 目的：建立食品过敏原牛奶成分LAMP(loop-mediated isothermal amplification)检测方法，并与实时荧光PCR(real-time PCR)检测方法比对。方法针对牛线粒体细胞色素b(cyt-b)基因设计LAMP引物并建立反应体系，在特异性和灵敏度方面与real-time PCR检测方法比对。结果本研究建立的LAMP方法检测9份不同品牌的牛奶和羊奶及其加工制品，没有出现交叉反应，具有良好的特异性。该方法的检测灵敏度为0.5%，与real-time PCR方法检测灵敏度相当。检测了69份实际样品，检测结果与real-time PCR检测结果一致。结论本研究建立的食品过敏原牛奶成分LAMP检测方法简单经济，检测结果可靠，可有效缩短检测时间，适用于过敏原牛奶成分的检测，具有良好的应用前景。 |
| Abstract_FL | Objective The loop-mediated isothermal amplification (LAMP) detection method was established for milk allergen testing and compared with the real-time PCR detection methods. Methods The cyt-b gene of bovine was used to design LAMP primers and then establish reaction system. The spe-cificity and sensitivity of LAMP were compared with real-time PCR detection method. Results The specificity of LAMP method was tested by 9 different milk and goat milk products. The results showed that the LAMP method was highly specific to milk. No cross-reaction was founded. The detection limit of LAMP reached 0.5%in base-material addition test which was consistent with the real-time PCR method. Through 69 practical food samples testing, the LAMP results were consistent with real-time PCR results. Conclusion The LAMP detection method of milk allergen established in this study is simple, economi-cal and reliable, which can effectively reduce the detection time and apply to the detection of milk aller-gen with good application prospects. |
| Author | 程晋霞 曾静 马丹 魏海燕 张海予 |
| AuthorAffiliation | 北京出入境检验检疫局，北京 100026 北京农学院，北京 102206 北京出入境检验检疫局,北京,100026 北京农学院,北京,102206 |
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| Author_FL | ZENG Jing ZHANG Hai-Yu WEI Hai-Yan MA Dan CHENG Jin-Xia |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Establishment of loop-mediated isothermal amplification method for detection of milk allergen in foods |
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| Keywords | allergen 牛奶 环介导等温扩增(LAMP) milk 实时荧光PCR real-time PCR loop-mediated isothermal amplification 过敏原 |
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| Publisher | 北京农学院，北京 102206%北京出入境检验检疫局,北京,100026%北京农学院,北京,102206 北京出入境检验检疫局，北京 100026 |
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| Snippet | 目的：建立食品过敏原牛奶成分LAMP(loop-mediated isothermal amplification)检测方法，并与实时荧光PCR(real-time PCR)检测方法比对。方法针对牛线粒体细胞色素b(cyt-b)基因设计LAMP引物并建立反应体系，在特异性和灵敏度方面与real-time... |
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| SubjectTerms | 实时荧光PCR 牛奶 环介导等温扩增(LAMP) 过敏原 |
| Title | 食品中牛奶过敏原成分LAMP检测方法的建立 |
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