Application of RNA interference for inhibiting the replication of feline immunodeficiency virus in chronically infected cell lines

RNA interference (RNAi) is a process in which double-stranded RNA induces the post-transcriptional sequence-specific degradation of homologous messenger RNA. The present study was carried out to apply the RNAi technology to inhibit the replication of feline immunodeficiency virus (FIV). Four small i...

Full description

Saved in:
Bibliographic Details
Published inVeterinary microbiology Vol. 120; no. 3; pp. 207 - 216
Main Authors Baba, Kenji, Mizukoshi, Fuminori, Goto-Koshino, Yuko, Setoguchi-Mukai, Asuka, Fujino, Yasuhito, Ohno, Koichi, Tsujimoto, Hajime
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 10.03.2007
Elsevier Science
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:RNA interference (RNAi) is a process in which double-stranded RNA induces the post-transcriptional sequence-specific degradation of homologous messenger RNA. The present study was carried out to apply the RNAi technology to inhibit the replication of feline immunodeficiency virus (FIV). Four small interfering RNAs (siRNAs) homologous to the FIV gag gene were synthesized and transfected into a feline fibroblastic cell line chronically infected with FIV (CRFK/FIV). These synthetic siRNAs efficiently inhibited the replication of FIV. Next, we examined the effect of retroviral vector-mediated transfer of FIV-specific short hairpin RNA (shRNA) on the replication of FIV in a feline T-cell line chronically infected with FIV (FL4). The retroviral vector-mediated transfer of FIV-specific shRNA was shown to markedly inhibit the replication of FIV in the FL4 cells. These results provide useful information for the development of RNAi-based gene therapy strategy to control FIV infection.
Bibliography:http://dx.doi.org/10.1016/j.vetmic.2006.10.033
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2006.10.033