Starch storage in the stems of wheat plants: localization and temporal changes

• Published in: Annals of Botany Vol. 103; no. 6; pp. 859 - 868
• Main Authors: Scofield, Graham N, Ruuska, Sari A, Aoki, Naohiro, Lewis, David C, Tabe, Linda M, Jenkins, Colin L.D
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2009; Oxford University Press (OUP); Oxford Publishing Limited (England)
• Subjects: Carbohydrate partitioning; early development; filling period; Flowering; Fructans; Grains; growth & development; Internodes; Leaves; metabolism; Nitrogen; Original; Parenchyma; Peduncle; Plant Stems; Plant Stems - metabolism; Plants; Starch; Starch - metabolism; starch granules; Starches; Stems; storage parenchyma; temporal variation; Tissue samples; Triticum; Triticum - growth & development; Triticum - metabolism; Triticum aestivum; Wheat; wheat starch; wheat stem; starch; Carbohydrate partitioning; storage parenchyma; wheat stem; peduncle
• ISSN: 0305-7364; 1095-8290; 1095-8290
• DOI: 10.1093/aob/mcp010

BACKGROUND AND AIMS: Carbohydrate temporarily accumulates in wheat stems during the early reproductive growth phase, predominantly as water soluble carbohydrate (WSC), and is subsequently remobilized during grain filling. Starch has also been reported as a minor storage carbohydrate component in wheat stems, but the details are lacking. METHODS: The accumulation and localization of starch in wheat stem and leaf sheath tissue over a developmental period from 6 d before anthesis to 35 d after anthesis was investigated. KEY RESULTS: The region of the peduncle enclosed by the flag-leaf sheath, and the penultimate internode were the main tissues identified as containing starch, in which the starch grains localized to the storage parenchyma cells. In contrast, the exposed peduncle lacked starch grains. Starch grains were also found in the flag-leaf and second-leaf sheath. Plants grown in low-nitrogen conditions exhibited increased storage of both starch and WSC compared with plants grown in high-nitrogen supply. CONCLUSIONS: The major accumulation and decrease of starch occurred temporally independently to that for WSC, suggesting a different functional role for starch in wheat stems. Starch reutilization concomitant with peduncle growth, and the early development of the reproductive structures, suggested a role in provision of energy and/or carbon scaffolds for these growth processes.