Mycobacterium pseudoshottsii Isolated from 24 Farmed Fishes in Western Japan

• Published in: Journal of Veterinary Medical Science Vol. 74; no. 2; pp. 275 - 278
• Main Authors: NAKANAGA, Kazue, HOSHINO, Yoshihiko, HATTORI, Yoko, YAMAMOTO, Atsushi, WADA, Shinpei, HATAI, Kishio, MAKINO, Masahiko, ISHII, Norihisa
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2012; Japan Science and Technology Agency
• Subjects: Animals; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Base Sequence; Chaperonin 60 - chemistry; Chaperonin 60 - genetics; Disease Outbreaks - veterinary; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Fish Diseases - epidemiology; Fish Diseases - microbiology; Japan - epidemiology; lethal fish infection; Marine; Molecular Sequence Data; Mycobacterium; Mycobacterium - genetics; Mycobacterium - isolation & purification; Mycobacterium Infections - epidemiology; Mycobacterium Infections - microbiology; Mycobacterium Infections - veterinary; Mycobacterium pseudoshottsii; mycolactone; Perciformes; Pisces; Polymerase Chain Reaction - veterinary; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; Sequence Alignment; Sequence Analysis, DNA; Japan; INW, Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.11-0226

Mycobacteria isolated from epizootics of farmed fishes in western Japan were examined for the first time using multigenotypic analysis. By analysis of the sequences of the internal transcribed spacer between the 16S and 23S rRNA genes (ITS) region and the partial 16S rRNA, hsp65 and rpoB genes, M. pseudoshottsii was identified as the causative agent in these infections. Prior to this study, only M. marinum has been known as the causative agent of lethal mycobacterial disease in marine fishes in Japan.