Enhancement of co-production of lutein and protein in Chlorella sorokiniana FZU60 using different bioprocess operation strategies

• Published in: Bioresources and bioprocessing Vol. 8; no. 1; p. 82
• Main Authors: Ma, Ruijuan, Zhang, Zhen, Tang, Zhuzhen, Ho, Shih-Hsin, Shi, Xinguo, Liu, Lemian, Xie, Youping, Chen, Jianfeng
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 30.08.2021; Springer Nature B.V; SpringerOpen
• Subjects: Algae; Aquatic microorganisms; Batch culture; Biochemical Engineering; Chemistry; Chemistry and Materials Science; Chlorella; Chlorella sorokiniana; Co-production; Continuous production; Cultivation; Environmental Engineering/Biotechnology; Fed batch; Industrial and Production Engineering; Light intensity; Luminous intensity; Lutein; Metabolites; Protein; Proteins; Strategy; Lutein; Strategy; Co-production; Protein; Chlorella sorokiniana
• ISSN: 2197-4365; 2197-4365
• DOI: 10.1186/s40643-021-00436-9

Co-production of multiple compounds is an efficient approach to enhance the economic feasibility of microalgae-based metabolites production. In this study, Chlorella sorokiniana FZU60 was cultivated under different bioprocess strategies to enhance the co-production of lutein and protein. Results showed that both lutein and protein content (7.72 and 538.06 mg/g, respectively) were highest at the onset of nitrogen deficiency under batch cultivation. Semi-batch III strategy, with 75% microalgal culture replacement by fresh medium, obtained similar content, productivity, and yield of lutein and protein as batch cultivation, demonstrating that it can be used for stable and continuous production. Fed-batch II strategy, feeding with 1/3 modified BG11 medium, achieved super-high lutein and protein yield (28.81 and 1592.77 mg/L, respectively), thus can be used for high-output production. Besides, two-stage strategy, combining light intensity shift and semi-batch cultivation, gained extremely high lutein and protein productivity (15.31 and 1080.41 mg/L/day, respectively), thereby is a good option for high-efficiency production. Moreover, the fed-batch II and two-stage strategy achieved high-quality lutein and protein, thus are promising for the co-production of lutein and protein in C. sorokiniana FZU60 for commercial application.