Evaluation of immunogenicity and protective efficacy of orally delivered Shigella type III secretion system proteins IpaB and IpaD

• Published in: Vaccine Vol. 31; no. 28; pp. 2919 - 2929
• Main Authors: Heine, Shannon J, Diaz-McNair, Jovita, Martinez-Becerra, Francisco J, Choudhari, Shyamal P, Clements, John D, Picking, Wendy L, Pasetti, Marcela F
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 19.06.2013; Elsevier
• Subjects: Administration, Intranasal; Administration, Oral; Allergy and Immunology; animal models; Animals; Antibodies, Bacterial - analysis; antigens; Antigens, Bacterial - administration & dosage; Antigens, Bacterial - immunology; Applied microbiology; Bacterial Proteins - administration & dosage; Bacterial Proteins - immunology; Bacterial Toxins - administration & dosage; Bacterial Toxins - immunology; Bacteriology; Biological and medical sciences; blood serum; Cytokines - immunology; Dysentery, Bacillary - immunology; Dysentery, Bacillary - prevention & control; Enterotoxins - administration & dosage; Enterotoxins - immunology; Escherichia coli; Escherichia coli Proteins - administration & dosage; Escherichia coli Proteins - immunology; Female; Fundamental and applied biological sciences. Psychology; heat; humans; immune response; Immunity, Mucosal; immunization; immunoglobulin A; immunoglobulin G; interleukin-10; interleukin-17; interleukin-2; interleukin-5; lungs; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; morbidity; mortality; mutants; oral administration; Oral immunization; pathogens; plasmids; Shigella; Shigella flexneri - immunology; Shigella vaccines; Shigella Vaccines - administration & dosage; Shigella Vaccines - immunology; shigellosis; spleen; Type III secretion proteins; type III secretion system; Vaccination; vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Subunit - administration & dosage; Vaccines, Subunit - immunology; invasion plasmid antigens; Shigella vaccines; nasopharynx-associated lymphoid tissues; TTSS; LT; ELISA unit; TNF; LPS; IFN; ASC; type III secretion system; antibody secreting cells; heat-labile enterotoxin; Ipas; enzyme-linked immunosorbent spot; interleukin; Oral immunization; MSD; NALT; Type III secretion proteins; orogastric; IL; i.n; o.g; interferon; BAL; EU; tumor necrosis factor; enzyme-linked immunosorbent assay; double mutant of the heat-labile enterotoxin; intranasal; SEM; standard error of the mean; lipopolysaccharide; ELIspot; Meso Scale Discovery; bronchoalveolar lavage; dmLT; ELISA; Immunization; Immunogenicity; Efficiency; Bacteria; Vaccine; Type III secretion system; Protein; Shigella; Enterobacteriaceae
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2013.04.045

Highlights • Mice were immunized orally with Shigella TTSS proteins IpaB/IpaD and E. coli dmLT. • Vaccine-specific immune responses and moderate protection were observed. • Compared side-by-side, the nasal route was more effective than the oral route. • IgA secreting cells and IL-2, IL-5 and IL-10 were associated with protection.