抗菌肽Protegrin-1基因的PCR扩增和克隆及序列分析
从杜长嘉猪的骨髓中提取基因组RNA，用反转录一聚合酶链式反应(RT—PCR)扩增抗茵肽Prote—grin-1(PPG-1)基因，获得一条565bp的片断，以pGEM—TEasyvector为栽体，将该基因片断克隆到大肠杆菌中．从筛选到的阳性克隆中分离出Protegrin一1基因，测定其序列，并由此推导出氨基酸序列．结果表明：①该片断长565bp，为Protegrin-1基因cDNA的部分序列，序列同源性比较表明，该基因序列与GeneBank登录的Protegrin-1基因序列具有较高的同源性，达到98．8％．②根据所测序列推导其成熟肽由18个氨基酸残基组成，其氨基酸序列与GeneBank登录...
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| Published in | Zhejiang daxue xue bao. Journal of Zhejiang University. Agriculture and life sciences. Nong ye yu sheng ming ke xue ban Vol. 30; no. 3; pp. 336 - 339 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江大学饲料科学研究所,浙江,杭州,310029
2004
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-9209 |
| DOI | 10.3321/j.issn:1008-9209.2004.03.022 |
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| Abstract | 从杜长嘉猪的骨髓中提取基因组RNA，用反转录一聚合酶链式反应(RT—PCR)扩增抗茵肽Prote—grin-1(PPG-1)基因，获得一条565bp的片断，以pGEM—TEasyvector为栽体，将该基因片断克隆到大肠杆菌中．从筛选到的阳性克隆中分离出Protegrin一1基因，测定其序列，并由此推导出氨基酸序列．结果表明：①该片断长565bp，为Protegrin-1基因cDNA的部分序列，序列同源性比较表明，该基因序列与GeneBank登录的Protegrin-1基因序列具有较高的同源性，达到98．8％．②根据所测序列推导其成熟肽由18个氨基酸残基组成，其氨基酸序列与GeneBank登录的Protegrin-1的成熟肽序列完全一致． |
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| AbstractList | 从杜长嘉猪的骨髓中提取基因组RNA，用反转录一聚合酶链式反应(RT—PCR)扩增抗茵肽Prote—grin-1(PPG-1)基因，获得一条565bp的片断，以pGEM—TEasyvector为栽体，将该基因片断克隆到大肠杆菌中．从筛选到的阳性克隆中分离出Protegrin一1基因，测定其序列，并由此推导出氨基酸序列．结果表明：①该片断长565bp，为Protegrin-1基因cDNA的部分序列，序列同源性比较表明，该基因序列与GeneBank登录的Protegrin-1基因序列具有较高的同源性，达到98．8％．②根据所测序列推导其成熟肽由18个氨基酸残基组成，其氨基酸序列与GeneBank登录的Protegrin-1的成熟肽序列完全一致． Q785; 从杜长嘉猪的骨髓中提取基因组RNA,用反转录-聚合酶链式反应(RT-PCR)扩增抗菌肽Protegrin-1(PPG-1)基因,获得一条565 bp的片断,以pGEM-T Easy vector为载体,将该基因片断克隆到大肠杆菌中.从筛选到的阳性克隆中分离出Protegrin-1基因,测定其序列,并由此推导出氨基酸序列.结果表明:①该片断长565 bp,为Protegrin-1基因cDNA的部分序列,序列同源性比较表明,该基因序列与GeneBank登录的Protegrin-1基因序列具有较高的同源性,达到98.8%.②根据所测序列推导其成熟肽由18个氨基酸残基组成,其氨基酸序列与GeneBank登录的Protegrin-1的成熟肽序列完全一致. |
| Author | 汪以真 刘光富 初晓娜 韩菲菲 |
| AuthorAffiliation | 浙江大学饲料科学研究所，浙江杭州310029 |
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| Snippet | ... Q785; 从杜长嘉猪的骨髓中提取基因组RNA,用反转录-聚合酶链式反应(RT-PCR)扩增抗菌肽Protegrin-1(PPG-1)基因,获得一条565 bp的片断,以pGEM-T Easy... |
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| StartPage | 336 |
| SubjectTerms | PPG-1 RT—PCR 基因克隆 序列分析 猪 骨髓 |
| Title | 抗菌肽Protegrin-1基因的PCR扩增和克隆及序列分析 |
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