东乡绿壳蛋鸡线粒体DNA控制区多态性及与9种地方乌鸡的进化分析

采用PCR产物直接测序的方法,对39只东乡绿壳蛋鸡（♂19、♀20）的线粒体DNA（mtDNA）控制区进行遗传多态性分析；并与9个国内其他地方乌鸡种的209个个体进行序列比对和进化分析.结果显示：东乡绿壳蛋鸡mtDNA控制区534 bp序列的G＋C含量为43.1％；序列共存在21个变异位点,其中转换占71.4％（15/21）、颠换占28.6％（6/21）；核苷酸多样度为0.00829±0.00130.分析10个乌鸡种的248个个体共获得73种单倍型,其中东乡绿壳蛋鸡含9种单倍型,单倍型多样度为0.773±0.048.品种间进化分歧显示：东乡绿壳蛋鸡与同处江西省的泰和丝羽乌骨鸡遗传距离最近（0....

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Published in浙江大学学报（农业与生命科学版） Vol. 40; no. 1; pp. 103 - 110
Main Author 章学东 李庆海 张成先 陈贤惠 楼立峰 王欢欢
Format Journal Article
LanguageChinese
Published 杭州市农业科学研究院,杭州,310024%浙江光大种禽业有限公司,浙江 海宁,314423 2014
Subjects
东乡绿壳蛋鸡
乌鸡
多态性
线粒体DNA
进化
线粒体DNA
evolutionary relatedness
Wugu chicken
mitochondrial DNA
Dongxiang blue-eggshell chicken
多态性
进化
polymorphism
东乡绿壳蛋鸡
乌鸡
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2013.06.012

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Summary:采用PCR产物直接测序的方法,对39只东乡绿壳蛋鸡（♂19、♀20）的线粒体DNA（mtDNA）控制区进行遗传多态性分析；并与9个国内其他地方乌鸡种的209个个体进行序列比对和进化分析.结果显示：东乡绿壳蛋鸡mtDNA控制区534 bp序列的G＋C含量为43.1％；序列共存在21个变异位点,其中转换占71.4％（15/21）、颠换占28.6％（6/21）；核苷酸多样度为0.00829±0.00130.分析10个乌鸡种的248个个体共获得73种单倍型,其中东乡绿壳蛋鸡含9种单倍型,单倍型多样度为0.773±0.048.品种间进化分歧显示：东乡绿壳蛋鸡与同处江西省的泰和丝羽乌骨鸡遗传距离最近（0.009）；湖北省的郧县乌骨鸡与云南省的盐津乌骨鸡、腾冲雪鸡距离最远（0.019、0.020）.结合邻接系统发生树发现,国内10个乌鸡种群体共可归纳出6大分支起源,其中,与红色原鸡（ Gallus gallus spadiceus）亚种同源的B分支包含的个体数最多（77/248,31.0％）,A分支其次（69/248,27.8％）；东乡绿壳蛋鸡含3个分支,A分支最多（25/39,64.1％）.本研究表明,东乡绿壳蛋鸡的遗传多态性呈中等；同时,国内各地方乌鸡种之间存在较为明显的地域关联性,而其母系来源则各有偏重.
Bibliography:Wugu chicken is a marvel of traditional Chinese poultry breeds and is characterized by black skin and bones , and grayish-black meat . There are nearly 17 species of Wugu chicken , not including breeds with partially black skin and bones , recorded in A nimal Genetic Resources in China published by the China National Commission of Animal Genetic Resources . Among these breeds , the Dongxiang blue-eggshell chicken was originally bred in Dongxiang County , Jiangxi Province , China . This breed is characterized by black feathers and most of the crest , beak , skin , muscle , bone , shins , and its toes are also black . The unique characteristic of the Dongxiang chicken is a blue eggshell . Mitochondrial DNA （ mtDNA） analysis has become an effective method to explore genetic relation among populations because of its low molecular mass , simple structure , rapid evolutionary rate , maternal inheritance , and non-tissue specification , among other features . The aim of the present study was to analyze mtDNA control
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2013.06.012