A comparison of the performance of mono- and bi-component electrospun conduits in a rat sciatic model

• Published in: Biomaterials Vol. 35; no. 32; pp. 8970 - 8982
• Main Authors: Cirillo, Valentina, Clements, Basak A, Guarino, Vincenzo, Bushman, Jared, Kohn, Joachim, Ambrosio, Luigi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.10.2014
• Subjects: Advanced Basic Science; Animals; Biocompatibility; Biomedical materials; Conduits; Dentistry; Disease Models, Animal; Electrospinning; Female; Ganglia, Spinal - cytology; Gelatin - chemistry; Gelatins; In vivo model; Muscle, Skeletal - physiology; Nerve Regeneration - drug effects; Nerves; Neurites - physiology; Organ Size - drug effects; Peripheral Nerve Injuries - therapy; Peripheral Nerves - cytology; Peripheral Nerves - drug effects; Polyesters - chemistry; Prostheses and Implants; Rats; Rats, Inbred Lew; Recovery of Function - drug effects; Regeneration; Sciatic Nerve - cytology; Sciatic Nerve - pathology; Sciatic nerve regeneration; Sciatic Neuropathy - therapy; Surgical implants; Tubular conduit; Electrospinning; In vivo model; Tubular conduit; Sciatic nerve regeneration
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2014.07.010

Abstract Synthetic nerve conduits represent a promising strategy to enhance functional recovery in peripheral nerve injury repair. However, the efficiency of synthetic nerve conduits is often compromised by the lack of molecular factors to create an enriched microenvironment for nerve regeneration. Here, we investigate the in vivo response of mono (MC) and bi-component (BC) fibrous conduits obtained by processing via electrospinning poly(ε-caprolactone) (PCL) and gelatin solutions. In vitro studies demonstrate that the inclusion of gelatin leads to uniform electrospun fiber size and positively influences the response of Dorsal Root Ganglia (DRGs) neurons as confirmed by the preferential extensions of neurites from DRG bodies. This behavior can be attributed to gelatin as a bioactive cue for the cultured DRG and to the reduced fibers size. However, in vivo studies in rat sciatic nerve defect model show an opposite response: MC conduits stimulate superior nerve regeneration than gelatin containing PCL conduits as confirmed by electrophysiology, muscle weight and histology. The G-ratio, 0.71 ± 0.07 for MC and 0.66 ± 0.05 for autograft, is close to 0.6, the value measured in healthy nerves. In contrast, BC implants elicited a strong host response and infiltrating tissue occluded the conduits preventing the formation of myelinated axons. Therefore, although gelatin promotes in vitro nerve regeneration, we conclude that bi-component electrospun conduits are not satisfactory in vivo due to intrinsic limits to their mechanical performance and degradation kinetics, which are essential to peripheral nerve regeneration in vivo.