Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons

• Published in: Nature neuroscience Vol. 24; no. 4; pp. 572 - 583
• Main Authors: Blum, Jacob A., Klemm, Sandy, Shadrach, Jennifer L., Guttenplan, Kevin A., Nakayama, Lisa, Kathiria, Arwa, Hoang, Phuong T., Gautier, Olivia, Kaltschmidt, Julia A., Greenleaf, William J., Gitler, Aaron D.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.04.2021; Nature Publishing Group
• Subjects: 38; 38/32; 45; 45/91; 631/337/2019; 631/378/2632/1664; Animal Genetics and Genomics; Animals; Autonomic Nervous System; Behavioral Sciences; Biological Techniques; Biomedical and Life Sciences; Biomedicine; Fluorescence; Gene expression; Gene sequencing; Health aspects; Heterogeneity; Identification and classification; Localization; Mice; Motor neurons; Motor Neurons - cytology; Motor task performance; Muscle, Skeletal - innervation; Muscles; Nervous system, Autonomic; Neurobiology; Neurons; Neurosciences; Nuclei (cytology); Physiological aspects; Resource; RNA sequencing; Single-Cell Analysis; Spatial discrimination; Spinal cord; Spinal Cord - cytology; Transcription; Transcriptome; Vertebrates; Viscera - innervation; United States
• ISSN: 1097-6256; 1546-1726; 1546-1726
• DOI: 10.1038/s41593-020-00795-0

The spinal cord is a fascinating structure that is responsible for coordinating movement in vertebrates. Spinal motor neurons control muscle activity by transmitting signals from the spinal cord to diverse peripheral targets. In this study, we profiled 43,890 single-nucleus transcriptomes from the adult mouse spinal cord using fluorescence-activated nuclei sorting to enrich for motor neuron nuclei. We identified 16 sympathetic motor neuron clusters, which are distinguishable by spatial localization and expression of neuromodulatory signaling genes. We found surprising skeletal motor neuron heterogeneity in the adult spinal cord, including transcriptional differences that correlate with electrophysiologically and spatially distinct motor pools. We also provide evidence for a novel transcriptional subpopulation of skeletal motor neuron (γ*). Collectively, these data provide a single-cell transcriptional atlas ( http://spinalcordatlas.org ) for investigating the organizing molecular logic of adult motor neuron diversity, as well as the cellular and molecular basis of motor neuron function in health and disease. Blum et al. performed single-nucleus RNA sequencing of the adult mouse spinal cord. This analysis revealed heterogeneity in the autonomic and skeletal motor systems and provides a resource to study motor neurons in health and disease.