Isolation, antimicrobial resistance and virulence characterization of Salmonella spp. from fresh foods in retail markets in Hangzhou, China

• Published in: PloS one Vol. 18; no. 10; p. e0292621
• Main Authors: Qian, Min, Xu, Dingting, Wang, Jiankang, Zaeim, Davood, Han, Jianzhong, Qu, Daofeng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.10.2023; Public Library of Science (PLoS)
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Biology and Life Sciences; Caco-2 Cells; Chickens - microbiology; China - epidemiology; Contamination; Disease susceptibility; Drug resistance in microorganisms; Drug Resistance, Bacterial - genetics; Drug Resistance, Multiple, Bacterial - genetics; Food; Foodborne Diseases - microbiology; Genetic transcription; Health aspects; Humans; Medical research; Medicine and Health Sciences; Medicine, Experimental; Mice; Microbial Sensitivity Tests; Prevalence studies (Epidemiology); Research and Analysis Methods; Safety and security measures; Salmonella; Salmonella food poisoning; Sulfonamides; Tetracycline; Tetracyclines; Vegetables; Virulence (Microbiology); Virulence - genetics; China; Iran
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0292621

Salmonella can cause severe foodborne diseases. This study investigated the prevalence of Salmonella spp. in fresh foods in Hangzhou market and their harborage of antibiotic resistance and virulence genes, antibiotic susceptibility, and pathogenicity. A total of 500 samples (pork, n = 140; chicken, n = 128; vegetable, n = 232) were collected over a one-year period. Salmonella was found in 4.2% (21) of samples with the detection rate in pork, chicken and vegetables as 4.3% (6), 6.3% (8), and 3% (7), respectively. One Salmonella strain was recovered from each positive sample. The isolates were identified as six serotypes, of which S. Enteritidis (n = 7) and S. Typhimurium (n = 6) were the most predominant serotypes. The majority of isolates showed resistance to tetracycline (85.7%) and/or ciprofloxacin (71.4%). Tetracycline resistance genes showed the highest prevalence (90.5%). The occurrence of resistance genes for β-lactams (blaTEM-1, 66.7%; and blaSHV, 9.5%) and aminoglycosides (aadA1, 47.6%; Aac(3)-Ia, 19%) was higher than sulfonamides (sul1, 42.9%) and quinolones (parC, 38.1%). The virulence gene fimA was detected in 57.1% of isolates. Gene co-occurrence analysis implied that resistance genes were associated with virulence genes. Furthermore, selected S. Typhimurium isolates (n = 4) carrying different resistance and virulence genes up-regulated the secretions of cytokines IL-6 and IL-8 by Caco-2 cells in different degrees, suggesting that virulence genes may play a role in inflammatory transcription. In in vivo virulence test, microbiological counts in mouse feces and tissues showed that all included S. Typhimurium were able to infect mice, with one strain showing significantly higher virulence than others. In conclusion, this study indicates Salmonella contamination in fresh foods in Hangzhou market poses a risk to public health and it should be closely monitored to prevent and control foodborne diseases.