Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities

• Published in: Nano convergence Vol. 8; no. 1; pp. 24 - 11
• Main Authors: Lee, Na Kyeong, Wang, Chi-Pin James, Lim, Jaesung, Park, Wooram, Kwon, Ho-Keun, Kim, Se-Na, Kim, Tae-Hyung, Park, Chun Gwon
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 16.08.2021; Springer Nature B.V; SpringerOpen; 나노기술연구협의회
• Subjects: Antibodies; Antibody Conjugation; Biocompatibility; Carbodiimide coupling; Carboxylic acids; Chemistry and Materials Science; Conjugation; Fragments; Glycolic acid; Immune system; Maleimide-thiol reaction; Materials Science; Nanoparticles; Nanoscale Science and Technology; Nanotechnology; Nanotechnology and Microengineering; Polyethylene glycol; Target drug delivery; 고분자공학; Nanoparticles; Antibody Conjugation; Target drug delivery; Maleimide-thiol reaction; Carbodiimide coupling
• ISSN: 2196-5404; 2196-5404
• DOI: 10.1186/s40580-021-00274-7

Antibodies have been widely used to provide targeting ability and to enhance bioactivity owing to their high specificity, availability, and diversity. Recent advances in biotechnology and nanotechnology permit site-specific engineering of antibodies and their conjugation to the surfaces of nanoparticles (NPs) in various orientations through chemical conjugations and physical adhesions. This study proposes the conjugation of poly(lactic- co -glycolic acid) (PLGA) NPs with antibodies by using two distinct methods, followed by a comparison between the cell-targeting efficiencies of both techniques. Full-length antibodies were conjugated to the PLGA-poly(ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) NPs through the conventional carbodiimide coupling reaction, and f(ab′) 2 antibody fragments were conjugated to the PLGA-poly(ethylene glycol)-maleimide(PLGA-PEG-Mal) NPs through interactions between the f(ab′) 2 fragment thiol groups and the maleimide located on the nanoparticle surface. The results demonstrate that the PLGA nanoparticles conjugated with the f(ab′) 2 antibody fragments had a higher targeting efficiency in vitro and in vivo than that of the PLGA nanoparticles conjugated with the full-length antibodies. The results of this study can be built upon to design a delivery technique for drugs through biocompatible nanoparticles.