Protective effect of berberine chloride against cisplatin-induced ototoxicity

• Published in: Genes & genomics Vol. 44; no. 1; pp. 1 - 7
• Main Authors: Kim, Jong-Heun, Baek, Jeong-In, Lee, In-Kyu, Kim, Un-Kyung, Kim, Ye-Ri, Lee, Kyu-Yup
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.01.2022; Springer; Springer Nature B.V; 한국유전학회
• Subjects: Animal Genetics and Genomics; Animals; Antimitotic agents; Antineoplastic agents; Antineoplastic Agents - adverse effects; Antioxidants; Antitumor agents; Apoptosis; Apoptosis - drug effects; Berberine; Berberine - chemistry; Berberine - pharmacology; Biomedical and Life Sciences; Cancer; Care and treatment; Caspase 3 - metabolism; Caspase-3; Cell survival; Cells, Cultured; Chlorides; Chlorides - chemistry; Chlorides - pharmacology; Cisplatin; Cisplatin - adverse effects; Cochlea; Cochlea - cytology; Cochlea - drug effects; Cochlea - metabolism; Colitis; Dementia disorders; Diabetes mellitus; DNA fragmentation; Explants; Hair cells; Hair Cells, Auditory - drug effects; Hair Cells, Auditory - metabolism; Health aspects; Human Genetics; Immunofluorescence; In Situ Nick-End Labeling; Life Sciences; Membrane Potential, Mitochondrial - drug effects; Mice; Microbial Genetics and Genomics; Mitochondria; Organ of Corti; Organ of Corti - cytology; Organ of Corti - drug effects; Organ of Corti - metabolism; Ototoxicity; Ototoxicity - etiology; Ototoxicity - metabolism; Ototoxicity - prevention & control; Oxidative stress; Plant Genetics and Genomics; Protective Agents - pharmacology; Reactive oxygen species; Reactive Oxygen Species - metabolism; Research Article; Vascular dementia; 생물학; Berberine chloride; Antioxidant; Cisplatin; ROS; Ototoxicity
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-021-01157-1

Background Cisplatin (CP) is an effective anticancer drug broadly used for various types of cancers, but it has shown ototoxicity that results from oxidative stress. Berberine has been reported for its anti-oxidative stress suggesting its therapeutic potential for many diseases such as colitis, diabetes, and vascular dementia. Objective Organ of Corti of postnatal day 3 mouse cochlear explants were used to compare hair cells after the treatment with cisplatin alone or with berberine chloride (BC) followed by CP. Methods We investigated the potential of the anti-oxidative effect of BC against the cisplatin-induced ototoxicity. We observed a reduced aberrant bundle of stereocilia in hair cells in CP with BC pre-treated group. Caspase-3 immunofluorescence and TUNEL assay supported the hypothesis that BC attenuates the apoptotic signals induced by CP. Reactive oxygen species level in the mitochondria were investigated by MitoSOX Red staining and the mitochondrial membrane potentials were compared by JC-1 assay. Results BC decreased ROS generation with preserved mitochondrial membrane potentials in mitochondria as well as reduced DNA fragmentation in hair cells. In summary, our data indicate that BC might act as antioxidant against CP by reducing the stress in mitochondria resulting in cell survival. Conclusion Our result suggests the therapeutic potential of BC for prevention of the detrimental effect of CP-induced ototoxicity.