Development of a highly efficient prime editor 2 system in plants

• Published in: Genome Biology Vol. 23; no. 1; p. 161
• Main Authors: Li, Juan, Chen, Like, Liang, Jing, Xu, Rongfang, Jiang, Yingli, Li, Yizhen, Ding, Jian, Li, Min, Qin, Ruiying, Wei, Pengcheng
• Format: Journal Article
• Language: English
• Published: London BioMed Central 25.07.2022; BMC
• Subjects: Binding sites; composite materials; Corn; Editors; Efficiency; Engineering; engineers; frequency; genome; Genomes; Mutation; Peptides; planting; Precise editing; Prime editing; Proteins; Rice; Short Report

Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA wi...