Development of a highly efficient prime editor 2 system in plants

Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA wi...

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Published inGenome Biology Vol. 23; no. 1; p. 161
Main Authors Li, Juan, Chen, Like, Liang, Jing, Xu, Rongfang, Jiang, Yingli, Li, Yizhen, Ding, Jian, Li, Min, Qin, Ruiying, Wei, Pengcheng
Format Journal Article
LanguageEnglish
Published London BioMed Central 25.07.2022
BMC
Subjects
Binding sites
composite materials
Corn
Editors
Efficiency
Engineering
engineers
frequency
genome
Genomes
Mutation
Peptides
planting
Precise editing
Prime editing
Proteins
Rice
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Summary:Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T 0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
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ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-022-02730-x