Development of a highly efficient prime editor 2 system in plants

Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA wi...

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Published inGenome Biology Vol. 23; no. 1; p. 161
Main Authors Li, Juan, Chen, Like, Liang, Jing, Xu, Rongfang, Jiang, Yingli, Li, Yizhen, Ding, Jian, Li, Min, Qin, Ruiying, Wei, Pengcheng
Format Journal Article
LanguageEnglish
Published London BioMed Central 25.07.2022
BMC
Subjects
Binding sites
composite materials
Corn
Editors
Efficiency
Engineering
engineers
frequency
genome
Genomes
Mutation
Peptides
planting
Precise editing
Prime editing
Proteins
Rice
Short Report
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Abstract Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T 0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
AbstractList Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T 0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T₀ rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
Abstract Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
ArticleNumber 161
Author Li, Yizhen
Xu, Rongfang
Li, Min
Jiang, Yingli
Wei, Pengcheng
Qin, Ruiying
Liang, Jing
Li, Juan
Ding, Jian
Chen, Like
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Cites_doi 10.1038/s41586-019-1711-4
10.1016/j.molp.2022.05.009
10.1016/j.cell.2021.09.018
10.1038/s41587-021-01039-7
10.1016/j.molcel.2021.12.002
10.1186/s13059-020-02170-5
10.1016/j.molp.2020.03.012
10.1038/s41477-021-01043-4
10.1038/s41587-022-01308-z
10.1111/pbi.13716
10.1038/s41556-020-0518-8
10.1038/srep25921
10.1016/j.xplc.2020.100043
10.1038/s41467-020-20314-w
10.1007/s11427-018-9402-9
10.1038/s41587-021-00868-w
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References AV Anzalone (2730_CR1) 2019; 576
Y-Y Jiang (2730_CR5) 2020; 21
Q Liu (2730_CR17) 2019; 62
R Xu (2730_CR15) 2022; 20
2730_CR19
L Hu (2730_CR16) 2016; 6
2730_CR18
G Liu (2730_CR2) 2022; 82
2730_CR14
W Xu (2730_CR4) 2020; 13
M Velimirovic (2730_CR10) 2021
2730_CR13
2730_CR20
Q Lin (2730_CR6) 2021; 39
X Zhang (2730_CR11) 2020; 22
W Xu (2730_CR7) 2022; 8
PJ Chen (2730_CR9) 2021; 184
M Song (2730_CR12) 2021; 12
JW Nelson (2730_CR8) 2022; 40
R Xu (2730_CR3) 2020; 1
References_xml – volume: 576
  start-page: 149
  year: 2019
  ident: 2730_CR1
  publication-title: Nature
  doi: 10.1038/s41586-019-1711-4
– ident: 2730_CR13
  doi: 10.1016/j.molp.2022.05.009
– volume: 184
  start-page: 5635
  year: 2021
  ident: 2730_CR9
  publication-title: Cell
  doi: 10.1016/j.cell.2021.09.018
– volume: 40
  start-page: 402
  year: 2022
  ident: 2730_CR8
  publication-title: Nature Biotechnology
  doi: 10.1038/s41587-021-01039-7
– volume: 82
  start-page: 333
  year: 2022
  ident: 2730_CR2
  publication-title: Molecular Cell
  doi: 10.1016/j.molcel.2021.12.002
– ident: 2730_CR20
– volume: 21
  start-page: 257
  year: 2020
  ident: 2730_CR5
  publication-title: Genome Biology
  doi: 10.1186/s13059-020-02170-5
– volume: 13
  start-page: 675
  year: 2020
  ident: 2730_CR4
  publication-title: Molecular plant
  doi: 10.1016/j.molp.2020.03.012
– volume: 8
  start-page: 45
  year: 2022
  ident: 2730_CR7
  publication-title: Nature Plants
  doi: 10.1038/s41477-021-01043-4
– ident: 2730_CR14
  doi: 10.1038/s41587-022-01308-z
– volume: 20
  start-page: 350
  year: 2022
  ident: 2730_CR15
  publication-title: Plant Biotechnology Journal
  doi: 10.1111/pbi.13716
– volume: 22
  start-page: 740
  year: 2020
  ident: 2730_CR11
  publication-title: Nature Cell Biology
  doi: 10.1038/s41556-020-0518-8
– ident: 2730_CR18
– ident: 2730_CR19
– volume: 6
  start-page: 25921
  year: 2016
  ident: 2730_CR16
  publication-title: Scientific Reports
  doi: 10.1038/srep25921
– volume: 1
  year: 2020
  ident: 2730_CR3
  publication-title: Plant Communications
  doi: 10.1016/j.xplc.2020.100043
– volume: 12
  start-page: 1
  year: 2021
  ident: 2730_CR12
  publication-title: Nature communications
  doi: 10.1038/s41467-020-20314-w
– volume: 62
  start-page: 1
  year: 2019
  ident: 2730_CR17
  publication-title: Science China Life Sciences
  doi: 10.1007/s11427-018-9402-9
– volume: 39
  start-page: 923
  year: 2021
  ident: 2730_CR6
  publication-title: Nature Biotechnology
  doi: 10.1038/s41587-021-00868-w
– volume-title: Barnum D, Yu T
  year: 2021
  ident: 2730_CR10
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Snippet Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2...
Abstract Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime...
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SubjectTerms Binding sites
composite materials
Corn
Editors
Efficiency
Engineering
engineers
frequency
genome
Genomes
Mutation
Peptides
planting
Precise editing
Prime editing
Proteins
Rice
Short Report
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Title Development of a highly efficient prime editor 2 system in plants
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