A step‐by‐step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited

• Published in: Applications in plant sciences Vol. 8; no. 4; pp. e11342 - n/a
• Main Authors: Windham, Michael D., Pryer, Kathleen M., Poindexter, Derick B., Li, Fay‐Wei, Rothfels, Carl J., Beck, James B.
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.04.2020; John Wiley and Sons Inc; Wiley
• Subjects: Angiospermae; chromosome number; chromosome squash; Chromosomes; cytology; Deoxyribonucleic acid; DNA; Ethanol; Flow cytometry; Flowering; genome; Genomes; Meiosis; Plant sciences; pollen; polyploidy; Protocol Note; Researchers; species complex; United States > US; chromosome number; cytology; chromosome squash; meiosis; species complex; polyploidy
• ISSN: 2168-0450; 2168-0450
• DOI: 10.1002/aps3.11342

Premise Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources—access to a microscope with 1000× magnification is the most limiting factor. Methods and Results Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.