Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii

• Published in: PloS one Vol. 16; no. 4; p. e0250524
• Main Authors: Masson, Florent, Rommelaere, Samuel, Marra, Alice, Schüpfer, Fanny, Lemaitre, Bruno
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.04.2021; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Bacterial Proteins - genetics; Biology and Life Sciences; Drosophila; Drosophila melanogaster - genetics; Drosophila melanogaster - immunology; Drosophila melanogaster - microbiology; Endosymbiosis; Female; Hemolymph - microbiology; Medicine and Health Sciences; Oogenesis - genetics; Proteome - genetics; Proteomics; Research and Analysis Methods; Risk factors; Signal Transduction - genetics; Signal Transduction - immunology; Spiroplasma; Spiroplasma - genetics; Spiroplasma - pathogenicity; Symbiosis - genetics; Symbiosis - immunology; Switzerland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0250524

Insects are frequently infected with heritable bacterial endosymbionts. Endosymbionts have a dramatic impact on their host physiology and evolution. Their tissue distribution is variable with some species being housed intracellularly, some extracellularly and some having a mixed lifestyle. The impact of extracellular endosymbionts on the biofluids they colonize (e.g. insect hemolymph) is however difficult to appreciate because biofluid composition can depend on the contribution of numerous tissues. Here we investigate Drosophila hemolymph proteome changes in response to the infection with the endosymbiont Spiroplasma poulsonii. S. poulsonii inhabits the fly hemolymph and gets vertically transmitted over generations by hijacking the oogenesis in females. Using dual proteomics on infected hemolymph, we uncovered a weak, chronic activation of the Toll immune pathway by S. poulsonii that was previously undetected by transcriptomics-based approaches. Using Drosophila genetics, we also identified candidate proteins putatively involved in controlling S. poulsonii growth. Last, we also provide a deep proteome of S. poulsonii, which, in combination with previously published transcriptomics data, improves our understanding of the post-transcriptional regulations operating in this bacterium.