Dark-field microscopy in imaging of plasmon resonant nanoparticles

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 124; pp. 111 - 117
• Main Authors: Liu, Mengmeng, Chao, Jie, Deng, Suhui, Wang, Kun, Li, Kun, Fan, Chunhai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2014
• Subjects: Biological; Breaking; Dark-field microscopy (DFM); Imaging; Localized surface plasmon resonance (LSPR); Microscopy; Microscopy - methods; Molecular dynamics; Nanoparticles; Nanoparticles - chemistry; Plasmonic nanoparticles; Plasmons; Scattering; Surface Plasmon Resonance - methods; Dark-field microscopy (DFM); Localized surface plasmon resonance (LSPR); Plasmonic nanoparticles
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2014.06.001

•Improvements of dark-field microscope (DFM).•Applications of DFM in LSPR imaging.•Applications of DFM in imaging and spectra analysis of plasmonic nanoparticles. Dark-field microscopy (DFM) and spectroscopy base on localized surface plasmon resonance (LSPR) have been widely applied in biological sensing and single-molecule imaging. Using plasmonic nanoparticles with controlled geometrical, optical, and surface chemical properties as the probes, the scattering light depending on the surrounding environment can be detected by DF microscope. Signal-to-noise radio and time resolution of the conventional DFM is not sufficient to identify single molecular dynamics. To break these limitations, significant improvements have been made in recent years. This critical review is focused on the developments of the DFM and the utilization of DFM as a powerful technology in the application of LSPR detection.