Antibody-Directed Urokinase: A Specific Fibrinolytic Agent
A specific fibrinolytic agent was synthesized by covalently coupling urokinase to a monoclonal antibody that was fibrin-specific and did not cross-react with fibrinogen. The antibody was raised against a synthetic peptide representing the seven amino-terminal residues of the beta chain of human fibr...
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Published in | Science (American Association for the Advancement of Science) Vol. 229; no. 4715; pp. 765 - 767 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
The American Association for the Advancement of Science
23.08.1985
American Association for the Advancement of Science |
Subjects | |
Online Access | Get full text |
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Summary: | A specific fibrinolytic agent was synthesized by covalently coupling urokinase to a monoclonal antibody that was fibrin-specific and did not cross-react with fibrinogen. The antibody was raised against a synthetic peptide representing the seven amino-terminal residues of the beta chain of human fibrin. The urokinaseantifibrin conjugate retained the original binding specificity of the antibody and showed 100-fold increased fibrinolysis in vitro when compared to unmodified urokinase. The presence of human fibrinogen at plasma concentration did not influence these properties. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.4023710 |