高盐辣椒坯中一株耐盐乳杆菌的分离及鉴定
目的：采用选择性的 MRS培养基，从湖南地区特色的盐坯辣椒中分离筛选出耐盐优势菌。方法得到一株能在18%NaCl培养基中生长的耐盐菌进行了生理生化实验，通过菌落PCR扩增其16S rDNA基因序列并测序，经过序列比对构建系统发育树。结果这株菌有较好的产乳酸、耐盐的能力，经过形态观察和生理生化试验验证，属于乳酸杆菌。结论经过16S rDNA进一步鉴定为植物乳杆菌。...
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| Published in | 食品安全质量检测学报 no. 4; pp. 1016 - 1020 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖南省发酵食品工程技术研究中心，长沙 410128%湖南农业大学食品科学技术学院,长沙,410128
2014
湖南农业大学食品科学技术学院，长沙 410128 食品科学与生物技术湖南省重点实验室，长沙 410128%食品科学与生物技术湖南省重点实验室，长沙 410128 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的：采用选择性的 MRS培养基，从湖南地区特色的盐坯辣椒中分离筛选出耐盐优势菌。方法得到一株能在18%NaCl培养基中生长的耐盐菌进行了生理生化实验，通过菌落PCR扩增其16S rDNA基因序列并测序，经过序列比对构建系统发育树。结果这株菌有较好的产乳酸、耐盐的能力，经过形态观察和生理生化试验验证，属于乳酸杆菌。结论经过16S rDNA进一步鉴定为植物乳杆菌。 |
|---|---|
| AbstractList | 目的：采用选择性的 MRS培养基，从湖南地区特色的盐坯辣椒中分离筛选出耐盐优势菌。方法得到一株能在18%NaCl培养基中生长的耐盐菌进行了生理生化实验，通过菌落PCR扩增其16S rDNA基因序列并测序，经过序列比对构建系统发育树。结果这株菌有较好的产乳酸、耐盐的能力，经过形态观察和生理生化试验验证，属于乳酸杆菌。结论经过16S rDNA进一步鉴定为植物乳杆菌。 |
| Abstract_FL | Objective To isolate some salt-tolerance strains with different colony morphologies from Hunan characteristic fermented chilli samples using selected MRS medium. Methods One isolated strain which could grow on an 18%NaCl salt tolerant bacteria medium was identified base on the physiological and biochemical experiments with 16S rDNA sequencing and phylogenetic tree analyses. Results This strain had a good ability of producing lactic acid and high tolerance to salt. Conclusion This strain was identified as Lactobacillus plantarum through 16S rDNA sequence analysis. |
| Author | 李梓铭 谢靓 蒋立文 李云倩 |
| AuthorAffiliation | 湖南农业大学食品科学技术学院，长沙 410128 食品科学与生物技术湖南省重点实验室，长沙 410128 食品科学与生物技术湖南省重点实验室，长沙 410128 湖南省发酵食品工程技术研究中心，长沙 410128 湖南农业大学食品科学技术学院,长沙,410128 |
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| Author_FL | LI Yun-Qian LI Zi-Ming JIANG Li-Wen XIE Jing |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Isolation and identification of salt-tolerance lactic acid bacterium from high-salt chili |
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| Title | 高盐辣椒坯中一株耐盐乳杆菌的分离及鉴定 |
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