Kinetic Flow-injection Determination of Hydrogen Peroxide by Use of Iron(III)-catalyzed Coloration and Its Application to the Determination of Biological Substances

A kinetic flow-injection (FI) method is described for the determination of hydrogen peroxide. This method is based on an iron(III)-catalyzed oxidative coupling of 4-aminoantipyrine with N,N-dimethylaniline by hydrogen peroxide. By measuring the change in the absorbance of the dye formed at 560 nm, 1...

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Published inAnalytical Sciences Vol. 17; no. 2; pp. 255 - 258
Main Authors MAEUCHIHARA, Norie, NAKANO, Shigenori, KAWASHIMA, Takuji
Format Journal Article
LanguageEnglish
Published Tokyo The Japan Society for Analytical Chemistry 2001
Japan Society for Analytical Chemistry
Japan Science and Technology Agency
Subjects
Ampyrone - chemistry
Analytical chemistry
Aniline Compounds - chemistry
Blood Glucose - analysis
Calibration
Catalysis
Chemistry
Exact sciences and technology
Flow Injection Analysis
Glucose Oxidase - chemistry
Humans
Hydrogen Peroxide - analysis
Indicators and Reagents
Iron
Kinetics
Photometry
Spectrometric and optical methods
Urate Oxidase - chemistry
Uric Acid - blood
Biological fluid
Catalytic reaction
Chemical analysis
Hydrogen peroxide
Interelement effect
Kinetic method
Visible spectrometry
Oxidative coupling
Visual colorimetry
Environmental materials
Analytical reagent
Quantitative analysis
Flow injection
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Summary:A kinetic flow-injection (FI) method is described for the determination of hydrogen peroxide. This method is based on an iron(III)-catalyzed oxidative coupling of 4-aminoantipyrine with N,N-dimethylaniline by hydrogen peroxide. By measuring the change in the absorbance of the dye formed at 560 nm, 1 × 10-6 - 6 × 10-4 M hydrogen peroxide could be determined with a sampling rate of 15 h-1. The relative standard deviation (n = 30) was 0.8% for 5 × 10-5 M hydrogen peroxide. There was little interference of the co-existing ions and compounds. After introducing some immobilized enzyme reactors to the FI system, the proposed method allowed the determination of glucose and uric acid ranging from 1 × 10-6 to 6 × 10-4 M with relative standard deviations of below 2%. The applicability of the method was demonstrated by determining these substances in serum samples.
Bibliography:ObjectType-Article-1
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content type line 23
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.17.255