Trypanosoma cruzi Proteome

• Published in: Science (American Association for the Advancement of Science) Vol. 309; no. 5733; pp. 473 - 476
• Main Authors: Atwood, J. A, Weatherly, D. B, Minning, T. A, Bundy, B, Cavola, C, Opperdoes, F. R, Orlando, R, Tarleton, R. L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 15.07.2005; The American Association for the Advancement of Science
• Subjects: Adaptation, Physiological; Amastigotes; Analysis; Animals; Antigens, Protozoan - analysis; Biochemistry; Biochemistry. Physiology. Immunology. Molecular biology; Biological and medical sciences; Causes of; Chromatography, Liquid; Computational Biology; Databases, Genetic; DNA; Energy Metabolism; Enzymes; Enzymes - genetics; Enzymes - metabolism; Epimastigotes; Fundamental and applied biological sciences. Psychology; Genes, Protozoan; Genetic aspects; Genome, Protozoan; Genomes; Genomics; Glycoproteins - analysis; Glycoproteins - genetics; Health aspects; Histidine - metabolism; Insect carriers of disease; Insect vectors; Insects as carriers of disease; Life Cycle Stages; Life cycles; Mass Spectrometry; Membrane Proteins - analysis; Membrane Proteins - genetics; Mucins - analysis; Multigene Family; Neuraminidase - analysis; Neuraminidase - genetics; Parasite hosts; Parasites; Peptides - analysis; Protein Transport; Proteins; Proteome; Proteomes; Proteomics; Protozoa; Protozoan Proteins - analysis; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Protozoans; Tropical diseases; Trypanosoma cruzi; Trypanosoma cruzi - chemistry; Trypanosoma cruzi - genetics; Trypanosoma cruzi - growth & development; Trypanosoma cruzi - metabolism; Trypanosomatina; Trypomastigotes; Belgium; United States; Kinetoplastida; Protozoa; Life history; Gene; Pathogenic; Parasite; Proteome; Developmental stage; Trypanosoma cruzi
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1110289

To complement the sequencing of the three kinetoplastid genomes reported in this issue, we have undertaken a whole-organism, proteomic analysis of the four life-cycle stages of Trypanosoma cruzi. Peptides mapping to 2784 proteins in 1168 protein groups from the annotated T. cruzi genome were identified across the four life-cycle stages. Protein products were identified from >1000 genes annotated as "hypothetical" in the sequenced genome, including members of a newly defined gene family annotated as mucin-associated surface proteins. The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes.