Brown adipose tissue thermogenesis heats brain and body as part of the brain-coordinated ultradian basic rest-activity cycle

• Published in: Neuroscience Vol. 164; no. 2; pp. 849 - 861
• Main Authors: Ootsuka, Y, de Menezes, R.C, Zaretsky, D.V, Alimoradian, A, Hunt, J, Stefanidis, A, Oldfield, B.J, Blessing, W.W
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.12.2009
• Subjects: Adipose Tissue, Brown - physiology; Animals; arterial blood pressure; Behavior, Animal - physiology; Blood Pressure - physiology; Body Temperature; Body Temperature Regulation - physiology; Brain - physiology; Circadian Rhythm - physiology; Female; Food Deprivation - physiology; Heart Rate - physiology; hippocampal theta rhythm; Hippocampus - physiology; Male; Neurology; Periodicity; Rats; Rats, Sprague-Dawley; Regional Blood Flow - physiology; Rest - physiology; sleep; temperature; Thermogenesis - physiology; thermoregulation; Theta Rhythm; wavelet mathematics; DWT; electroencephalograph; slow wave sleep; heart rate; discrete wavelet transforms; EEG; HR; thermoregulation; BRAC; rapid eye movement; SWS; AP; sleep; hippocampal theta rhythm; basic rest-activity cycle; arterial pressure; arterial blood pressure; wavelet mathematics; brown adipose tissue; BAT; temperature; REM
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2009.08.013

Abstract Brown adipose tissue (BAT), body and brain temperatures, as well as behavioral activity, arterial pressure and heart rate, increase episodically during the waking (dark) phase of the circadian cycle in rats. Phase-linking of combinations of these ultradian (<24 h) events has previously been noted, but no synthesis of their overall interrelationships has emerged. We hypothesized that they are coordinated by brain central command, and that BAT thermogenesis, itself controlled by the brain, contributes to increases in brain and body temperature. We used chronically implanted instruments to measure combinations of bat, brain and body temperatures, behavioral activity, tail artery blood flow, and arterial pressure and heart rate, in conscious freely moving Sprague–Dawley rats during the 12-h dark active period. Ambient temperature was kept constant for any particular 24-h day, varying between 22 and 27 °C on different days. Increases in BAT temperature (≥0.5 °C) occurred in an irregular episodic manner every 94±43 min (mean±SD). Varying the temperature over a wider range (18–30 °C) on different days did not change the periodicity, and neither body nor brain temperature fell before BAT temperature episodic increases. These increases are thus unlikely to reflect thermoregulatory homeostasis. Episodic BAT thermogenesis still occurred in food-deprived rats. Behavioral activity, arterial pressure (18±5 mmHg every 98±49 min) and heart rate (86±31 beats/min) increased approximately 3 min before each increase in BAT temperature. Increases in BAT temperature (1.1±0.4 °C) were larger than corresponding increases in brain (0.8±0.4 °C) and body (0.6±0.3 °C) temperature and the BAT episodes commenced 2–3 min before body and brain episodes, suggesting that BAT thermogenesis warms body and brain. Hippocampal 5–8 Hz theta rhythm, indicating active engagement with the environment, increased before the behavioral and autonomic events, suggesting coordination by brain central command as part of the 1–2 h ultradian basic rest-activity cycle (BRAC) proposed by Kleitman.