Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea

Chlamydia pecorum (designated 22–58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C. pecorum positive reactions in the jejunal villi. C. pecorum, designated 24–100, was...

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Published inJournal of Veterinary Medical Science Vol. 77; no. 7; pp. 777 - 782
Main Authors OHTANI, Akifumi, KUBO, Masahito, SHIMODA, Hiroshi, OHYA, Kenji, IRIBE, Tadashi, OHISHI, Daiki, ENDOH, Daiji, OMATSU, Tsutomu, MIZUTANI, Tetsuya, FUKUSHI, Hideto, MAEDA, Ken
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.2015
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
Animals
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Bacteriology
Cattle
Cattle Diseases - microbiology
Chlamydia - genetics
Chlamydia - immunology
Chlamydia Infections - immunology
Chlamydia Infections - microbiology
Chlamydia Infections - veterinary
Chlamydia pecorum
diarrhea
Diarrhea - microbiology
Diarrhea - veterinary
Female
Intestines - microbiology
Polymerase Chain Reaction - veterinary
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Summary:Chlamydia pecorum (designated 22–58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C. pecorum positive reactions in the jejunal villi. C. pecorum, designated 24–100, was isolated from the feces of a calf with diarrhea in another farm in Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers against C. pecorum was confirmed in paired sera. Nucleotide sequence identities of omp1 genes of the 2 isolates were 100%. The isolates were genetically and antigenically more closely related to C. pecorum Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from sheep with polyarthritis. These results indicate that C. pecorum strains similar to 22–58 and 24–100 might be endemic in Yamaguchi Prefecture and cause enteric disease in cattle.
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.14-0585