Oocyte mitochondrial bioenergy potential and oxidative stress: within-/between-subject, in vivo versus in vitro maturation, and age-related variations in a sheep model

• Published in: Fertility and sterility Vol. 97; no. 3; pp. 720 - 728.e1
• Main Authors: Martino, Nicola Antonio, Lacalandra, Giovanni Michele, Filioli Uranio, Manuel, Ambruosi, Barbara, Caira, Michele, Silvestre, Fabio, Pizzi, Flavia, Desantis, Salvatore, Accogli, Gianluca, Dell’Aquila, Maria Elena
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2012; Elsevier
• Subjects: adults; Age Factors; Aging - metabolism; Animals; antioxidant activity; bioenergy; Biological and medical sciences; Biomarkers - metabolism; Cells, Cultured; donor age; energy; Energy Metabolism - drug effects; Estrus Synchronization; ewes; Female; Fertility Agents, Female - pharmacology; Gynecology. Andrology. Obstetrics; In Vitro Oocyte Maturation Techniques; Internal Medicine; intracellular ROS levels; Medical sciences; Metaphase; Mitochondria - drug effects; Mitochondria - metabolism; mitochondria distribution pattern and activity; mt/ROS colocalization; Obstetrics and Gynecology; Oocyte Retrieval; oocytes; Oocytes - drug effects; Oocytes - metabolism; Ovulation Induction; oxidative stress; Oxidative Stress - drug effects; prospective studies; reactive oxygen species; Reactive Oxygen Species - metabolism; Sheep; Sheep oocyte; spaying; intracellular ROS levels; mitochondria distribution pattern and activity; mt/ROS colocalization; Sheep oocyte; donor age; Oxidative stress; Free radical; Mitochondria; Meat animals; Ungulata; Age; Human; Oxygen; In vitro; Ripening; In vivo; Vertebrata; Mammalia; Donor; Distribution; Sheep; Models; Artiodactyla; Intracellular; Comparative study; Oocyte

To analyze within-/between-subject, in vivo versus in vitro maturation (IVM), and age-related variations of mitochondrial (mt) bioenergy potential and oxidative status of metaphase II (MII) oocytes recovered from hormonally stimulated sheep. Prospective study. Academic basic research laboratory. Ten...