Oocyte mitochondrial bioenergy potential and oxidative stress: within-/between-subject, in vivo versus in vitro maturation, and age-related variations in a sheep model

• Published in: Fertility and sterility Vol. 97; no. 3; pp. 720 - 728.e1
• Main Authors: Martino, Nicola Antonio, Lacalandra, Giovanni Michele, Filioli Uranio, Manuel, Ambruosi, Barbara, Caira, Michele, Silvestre, Fabio, Pizzi, Flavia, Desantis, Salvatore, Accogli, Gianluca, Dell’Aquila, Maria Elena
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2012; Elsevier
• Subjects: adults; Age Factors; Aging - metabolism; Animals; antioxidant activity; bioenergy; Biological and medical sciences; Biomarkers - metabolism; Cells, Cultured; donor age; energy; Energy Metabolism - drug effects; Estrus Synchronization; ewes; Female; Fertility Agents, Female - pharmacology; Gynecology. Andrology. Obstetrics; In Vitro Oocyte Maturation Techniques; Internal Medicine; intracellular ROS levels; Medical sciences; Metaphase; Mitochondria - drug effects; Mitochondria - metabolism; mitochondria distribution pattern and activity; mt/ROS colocalization; Obstetrics and Gynecology; Oocyte Retrieval; oocytes; Oocytes - drug effects; Oocytes - metabolism; Ovulation Induction; oxidative stress; Oxidative Stress - drug effects; prospective studies; reactive oxygen species; Reactive Oxygen Species - metabolism; Sheep; Sheep oocyte; spaying; intracellular ROS levels; mitochondria distribution pattern and activity; mt/ROS colocalization; Sheep oocyte; donor age; Oxidative stress; Free radical; Mitochondria; Meat animals; Ungulata; Age; Human; Oxygen; In vitro; Ripening; In vivo; Vertebrata; Mammalia; Donor; Distribution; Sheep; Models; Artiodactyla; Intracellular; Comparative study; Oocyte
• ISSN: 0015-0282; 1556-5653; 1556-5653
• DOI: 10.1016/j.fertnstert.2011.12.014

To analyze within-/between-subject, in vivo versus in vitro maturation (IVM), and age-related variations of mitochondrial (mt) bioenergy potential and oxidative status of metaphase II (MII) oocytes recovered from hormonally stimulated sheep. Prospective study. Academic basic research laboratory. Ten adult ewes. Estrus synchronization, controlled ovarian hyperstimulation (COH), ovariohysterectomy; follicular and oviductal oocyte retrieval; IVM of follicular oocytes. Mean ± SD, within-subject (CVw) and between-subject (CVb) variation coefficients of mt activity, intracellular reactive oxygen species (ROS) levels, and mt/ROS colocalization in sheep oocytes from young and aged donors and matured in vivo (in vivo MIIs) or in vitro (IVM MIIs). Within- and between-subject, in vivo versus IVM, and age-related variations of mt activity were observed in MII oocytes from hormonally stimulated donor sheep. ROS levels increased significantly in oocytes from aged donors. Mt-ROS colocalization was consistently higher in in vivo MIIs compared with IVM MIIs. Oviductal energy/antioxidant ability is influenced by COH. Oocyte energy/oxidative status is affected by within-/between-subject, in vivo versus IVM, and age-related variations. Mt/ROS colocalization is a reliable marker of in vivo MII oocytes.